Sökning: "Janne Lehtiö"

Hittade 5 avhandlingar innehållade orden Janne Lehtiö.

  1. 1. Functional studies and engineering of family 1 carbohydrate-binding modules

    Författare :Janne Lehtiö; KTH; []
    Nyckelord :Cellulose-binding; carbohydrate-binding modules; phage display; bacterial surface display; combinatorial protein library; protein engineering; Trichoderma reesei; Staphylococcus carnosus;

    Sammanfattning : The family 1 cellulose-binding modules (CBM1) form a groupof small, stable carbohydrate-binding proteins. These modulesare essential for fungal cellulosedegradation. This thesisdescribes both functional studies of the CBM1s as well asprotein engineering of the modules for several objectives. LÄS MER

  3. 2. Development and Application of Proximity Assays for Proteome Analysis in Medicine

    Författare :Felipe Marques Souza de Oliveira; Masood Kamali-Moghaddam; Ulf Landegren; Mats Gullberg; Janne Lehtiö; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Solid-phase proximity ligation assay; post-translational modifications; glycosylation; phosphorylation; Enzyme-linked immunosorbent assay; immunoassay and rolling circle amplification; Proximity Extension Assay; inflammation protein biomarkers; autoantibodies; autoimmune disease;

    Sammanfattning : Along with proteins, a myriad of different molecular biomarkers, such as post-translational modifications and autoantibodies, could be used in an attempt to improve disease detection and progression. In this thesis, I build on several iterations of the proximity ligation assay to develop and apply new adaptable methods to facilitate detection of proteins, autoantibodies and post-translational modifications. LÄS MER

  4. 3. Molecular Signatures of Neuropathic Pain : Revealing Pain-Related Signaling Processes in Spinal Cord Using Mass Spectrometric Methodologies

    Författare :Ping Sui; Jonas Bergquist; Konstantin Artemenko; Janne Lehtiö; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Mass spectrometry; Spinal cord; Neuropathic pain; Quantitative proteomics; Imaging mass spectrometry; Kemi med inriktning mot analytisk kemi; Chemistry with specialization in Analytical Chemistry;

    Sammanfattning : In this thesis, the detection of global proteomics alteration and changes in neuropeptide distribution caused by neuropathic pain in rat spinal cord tissue was the main focus. Neuropathic pain (NP) is a major clinical syndrome caused by disease or dysfunction of the nervous system and often mediated by neuronal networks in the spinal cord. LÄS MER

  5. 4. Multivariate profiling of metabolites in human disease : Method evaluation and application to prostate cancer

    Författare :Elin Thysell; Henrik Antti; Janne Lehtiö; Umeå universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; metabolite profiling; metabolomics; predictive metabolomics; mass spectrometry; GC-MS; biomarkers; chemometrics; design of experiments; multivariate data analysis; prostate cancer; bone metastases; plasma; biologisk kemi; biological chemistry;

    Sammanfattning : There is an ever increasing need of new technologies for identification of molecular markers for early diagnosis of fatal diseases to allow efficient treatment. In addition, there is great value in finding patterns of metabolites, proteins or genes altered in relation to specific disease conditions to gain a deeper understanding of the underlying mechanisms of disease development. LÄS MER

  7. 5. Multivariate analyses of proteomic and metabolomic patterns in brain tumors

    Författare :Carl Wibom; A Tommy Bergenheim; Henrik Antti; Mikael Johansson; Janne Lehtiö; Umeå universitet; []
    Nyckelord :glioblastoma; meningioma; proteomics; metabolomics; multivariate analyses; onkologi; Oncology;

    Sammanfattning : Glioblastoma multiforme (GBM) is the most common primary brain tumor. Given the current standard of care, the prognosis for patients diagnosed with this disease is still poor. There consequently exists a need to improve current treatments, as well as to develop new ones. LÄS MER