Sökning: "Industrial evolution"

Visar resultat 6 - 10 av 212 avhandlingar innehållade orden Industrial evolution.

  1. 6. Staphylococcal surface display in directed evolution

    Författare :Nina Kronqvist; Stefan Ståhl; Mats A. A. Persson; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; affibody; combinatorial library; directed evoluation; Gram-positive bacteria. protein engineering; Bioengineering; Bioteknik;

    Sammanfattning : Engineered affinity proteins have together with naturally derived antibodies becomeindispensable tools in many areas of life-science and with the increasing number ofapplications, the need for high-throughput methods for generation of such different affinityproteins is evident. Today, combinatorial protein engineering is the most successful strategy toisolate novel non-immunoglobulin affinity proteins. LÄS MER

  3. 7. Ribosome display for selection and evolution of affibody molecules

    Författare :Sebastian Grimm; Per-Åke Nygren; Mike Taussig; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; affibody; ribosome display; phage display; combinatorial protein engineering; library; murine IgG1; SATB1; Ras; Raf; Genetic engineering including functional genomics; Genteknik inkl. funktionsgenomik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

    Sammanfattning : Affinity proteins are invaluable tools in biotechnological and medical applications. This thesis is about combinatorial protein engineering principles for the generation of novel affinity proteins to purify mouse immunoglobulin, detect a potential cancer marker protein or inhibit a cell proliferation pathway. LÄS MER

  4. 8. Towards Classification and Functional Description of Enzymes: A case study of feruloyl esterases

    Författare :Gupta Udatha; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; descriptors; enzyme immobilization; catalytic triad; pharmacophore; enzyme promiscuity; functional classification; molecular docking; feruloyl esterases; protein evolution; structure-function relationship;

    Sammanfattning : The prediction of enzyme functionality from sequence or structure data remains a challenging task that can be best addressed by studying the structure-function relationships determined from previously available information. This thesis work was focused on developing a reliable classification and functional description for the feruloyl esterase (FAE) enzyme family, whose members’ possess both structural and catalytic promiscuity. LÄS MER

  5. 9. Robustness quantification in yeast: A methodology to study phenotypic, evolutionary, and genomic aspects of microbial robustness

    Författare :Cecilia Trivellin; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; bioproduction; Saccharomyces cerevisiae; adaptive laboratory evolution; High-throughput; fluctuating conditions; perturbations;

    Sammanfattning : Bioprocesses contribute to the shift towards a more sustainable economy. In bioprocesses, valuable chemicals can be generated from renewable resources while, at the same time, reducing carbon emissions. A major hurdle in bringing bio-based products to market is the time and cost involved in designing efficient cell factories. LÄS MER

  7. 10. Insights into the metabolism of Clostridium thermocellum for cellulosic ethanol production

    Författare :Johannes Yayo; Antonius J. A. van Maris; Richard Sparling; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Clostridium thermocellum; ethanol; glucose; fructose; amino acids; pyrophosphate; chaotropicity; thermodynamic driving force; laboratory evolution; chemostats; metabolic engineering; Biotechnology; Bioteknologi;

    Sammanfattning : The societal goal of reaching net-zero CO2 emissions requires development of integrated biorefineries to produce biomass-derived fuels and chemicals. For sustainable second-generation bioethanol production, consolidated bioprocessing with the thermophile Clostridium thermocellum is regarded as a promising concept in view of the microorganism’s native ability to efficiently degrade plant cell wall material. LÄS MER