Sökning: "yeast extract"

Visar resultat 1 - 5 av 23 avhandlingar innehållade orden yeast extract.

  1. 1. Catabolism of Pyrimidines in Yeast

    Författare :Anna Rasmussen; Molekylär cellbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Pyrimidine degradation; catabolism of pyrimidines; nucleic acid precursors; yeast; Lachancea kluyveri; URC pathway;

    Sammanfattning : Pyrimidines are important precursors for nucleic acids, and constantly synthesized and degraded in the cell to maintain a balanced supply. Many microoganisms can also extract the nitrogens from the pyrimidine ring, and use them as a nitrogen source when “preferred” sources are absent. LÄS MER

  3. 2. Impact of autocrine factors on physiology and productivity in Trichoplusia ni serum-free cultures

    Författare :Ulrika Eriksson; Lena Häggström; Per Berglund; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Trichoplusia ni; High Five; insect cells; BEVS; serum-free medium; yeast extract; conditioned medium; autocrine regulation of proliferation; growth factors; cell cycle; metalloproteinase; specific productivity; Bioteknik; Bioengineering; Bioteknik;

    Sammanfattning : The aim of this study was to increase the understanding of the mechanisms regulating cell proliferation and recombinant protein production in serum-free cultures of Trichoplusia ni (T. ni) insect cells.Conditioned medium (CM) was shown to contain both stimulatory and inhibitory factors (CM factors) influencing cell growth. LÄS MER

  4. 3. PCR-based detection of microorganisms in complex biological samples

    Författare :Pär-G Lantz; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Density gradient centrifugation; DNA extraction; Aqueous two-phase systems; Meat; Faeces; Cheese; Yeast; Helicobacter pylori; Yersinia enterocolitica; Listeria monocytogenes; PCR; Inhibition; Sample preparation; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Sammanfattning : The detection of microorganisms by PCR can be divided into four steps: (1) sample collection, (2) sample preparation, (3) DNA amplification and (4) detection of PCR products. The major problem in developing PCR-based detection methods is the sample preparation step. LÄS MER

  5. 4. Nutritional Limitations of a Green Protein Shift with Focus on Iron

    Författare :Cecilia Mayer Labba; Chalmers tekniska högskola; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; phytate; non-heme iron absorption; antinutrients; protein shift; protein extract; meat substitutes; plant-based; fava bean; zinc bioavailability; iron bioavailability; sustainable nutrition;

    Sammanfattning : A dietary shift into plant-based diets (PBD) to reduce the climate footprint is advocated. Effects on nutrition and health from a modern PBD, composed of replacement products based on protein extracts are however currently unknown. LÄS MER

  7. 5. Activation of human dendritic cells : role of the allergenic yeast Malassezia and NK cells

    Författare :Eva Buentke; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Antigen-presenting cells; atopic eczema dermatitis syndrome; cytokines; dendritic cells; Malassezia; NK cells; yeast;

    Sammanfattning : Immature bone marrow derived dendritic cells (DCs) patrol the periphery of our body, where they encounter and take up foreign antigens. Once activated, DCs migrate to regional lymph nodes to present peptides derived from these antigens to naive T cells, and elicit different T helper (Th) cell responses. LÄS MER