Sökning: "single vesicle imaging"

Visar resultat 1 - 5 av 11 avhandlingar innehållade orden single vesicle imaging.

  1. 1. Quantitative chemical imaging to study content release from single nanovesicles

    Författare :Tho D. K. Nguyen; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Exocytosis; Vesicle; Mass spectrometry imaging; Electron microscopy; Fluorescence microscopy; Electrochemical analysis; NanoSIMS;

    Sammanfattning : Cellular communication is vital for the survival of multicellular organisms. This process often relies on a highly regulated mechanism called exocytosis, which involves the release of chemical signals. During exocytosis, vesicles can fully or partially release their contents. LÄS MER

  3. 2. Fluorescence microscopy studies of interactions between monomeric α-Synuclein and lipid membranes

    Författare :Alexandra Andersson; NanoLund: Centre for Nanoscience; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Total internal reflection fluorescence microscopy; single vesicle imaging; single molecule imaging; protein-lipid interactions; α-Synuclein;

    Sammanfattning : α-Synuclein (α-Syn), is an intrinsically disordered protein present in the substantia nigra part of the brain. α-Syn is commonly known to associate with lipid membranes containing anionic lipids. LÄS MER

  4. 3. Correlative Chemical Imaging of Nanoscale Subcellular Structures

    Författare :Stefania Rabasco; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : Chemical imaging can elucidate complex mechanisms, relationships, and components of biological samples. For example, it can reveal properties such as chemical composition, chemical structure, reactivity, and topography. Several imaging techniques exist, each providing different types of information. LÄS MER

  5. 4. New Electrochemical Tools to Study Exocytosis

    Författare :Joakim Wigström; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; amperometry; QCM-D; micro electrode array; large dense core vesicles; chromaffin cells; PC-12 cells; microelectrode; exocytosis; electrochemical cytometry; electrochemical imaging; acetylcholine; endocytosis; nanoparticles; enzyme biosensor; microfabrication;

    Sammanfattning : The work described in this thesis has the focus on the development of new analytical tools to study processes related to cellular secretion (exocytosis) in cell models. Four novel techniques were developed, allowing new ways to study processes related to exocytosis, and gain previously unattainable knowledge. LÄS MER

  7. 5. Waveguide Evanescent-Field Microscopy for Label-Free Monitoring of Biological Nanoparticles: Fabrication, Characterization and Application

    Författare :Mokhtar Mapar; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Biosensor; Protein interaction; Waveguide scattering microscopy; Waveguide fluorescence microscopy; Supported lipid bilayer; Image processing; Lipid vesicle; Nanofabrication; Exosome;

    Sammanfattning : The recent development of microscopy methods, biological assays and bioanalytical sensors has significantly advanced the understanding of biological systems. Surface-based bioanalytical sensors have in recent years gained increased interest thanks to improvements in sensitivity and simplicity to use. LÄS MER