Sökning: "single cell gel electrophoresis assay"
Visar resultat 1 - 5 av 6 avhandlingar innehållade orden single cell gel electrophoresis assay.
1. Evolving the Methodology for Detection of Primary DNA Damage : Development, adaptation and assessment of the single cell gel electrophoresis (comet) assay
Sammanfattning : Deoxyribonucleic acid (DNA) is one of the most important molecules in nature. It is the fundamental carrier of evolutionary information and constitutes the genetic blueprint of all living organisms. Being the sole source of information, it is vital for the cell to transmit the correct genetic information from generation to generation. LÄS MER
2. Application of Padlock Probe Based Nucleic Acid Analysis In Situ
Sammanfattning : The great variation displayed by nucleic acid molecules in human cells, and the continuous discovery of their impact on life, consequently require continuous refinements of molecular analysis techniques. Padlock probes and rolling circle amplification offer single nucleotide discrimination in situ, a high signal-to-noise ratio and localized detection within cells and tissues. LÄS MER
3. Health of municipal sewage workers : Studies of cancer incidence, biomarkers of carcinogenicity and genotoxicity, and self reported symptoms
Sammanfattning : The occupational exposures of sewage workers are complex and variable, and include a great variety of biological and chemical agents. Previous research has focused mostly on infections and various symptoms among sewage workers, e.g. abdominal and respiratory symptoms. LÄS MER
4. Measurement of primary DNA damage in individual cells from humans and mice using the alkaline single cell gel electrophoresis assay
Sammanfattning : Primary DNA damage is considered to be an important step in the development of tumours and itmay also serve as a biomarker of genotoxic stress. Measurement of DNA strand breaks in peripherallymphocytes can yield useful information about the suitability of these cells for biomonitoring purposesand subsequently risk assessment. LÄS MER
5. Polyamines are involved in the regulation of S phase and DNA synthesis
Sammanfattning : Our research group has previously shown that treatment with the polyamine biosynthesis inhibitors a-difluoromethylornithine (DFMO) or amidinoindan-1-one 2´-amidinohydrazone (CGP 48664) inhibited S phase progression before any other cell cycle phase was affected. This study was undertaken to further investigate the role of polyamines in the regulation of S phase progression and DNA synthesis. LÄS MER
