Sökning: "proximity ligation"
Visar resultat 11 - 15 av 63 avhandlingar innehållade orden proximity ligation.
11. Development and Application of Triple Specific Proximity Ligation Assays (3PLA)
Sammanfattning : After the completion of the human genome project the human genome was annotated with the surprisingly small amount of 24 000 (www.ensemble.com) genes. LÄS MER
12. Making Visible the Proximity Between Proteins
Sammanfattning : Genomic DNA is the template of life - the entity which is characterized by a self-sustaining anatomical development, regulated signaling processes, the ability to reproduce and to respond to stimuli. Through what is classically known as the central dogma, the genome is transcribed into mRNA, which in turn is translated into proteins. LÄS MER
13. Ligation-mediated Molecular Analysis of Influenza Subtypes, Splicing and Protein Glycosylation
Sammanfattning : Binder-based assays are employed throughout the life sciences. Powerful signal amplification techniques have enabled detection of very rare molecule species diluted in simple buffers. Unspecific binding of primary binders leads to increased background in more complex samples. LÄS MER
14. Improvements and Applications of in situ Proximity Ligation Assays
Sammanfattning : The cells building up the human body is in constant communication with each other. This communication is done through large complex networks of signaling pathways for inter- and intracellular signal transduction. The signaling activity regulates many important processes, for example cell death, proliferation and differentiation. LÄS MER
15. Development and Application of Proximity Assays for Proteome Analysis in Medicine
Sammanfattning : Along with proteins, a myriad of different molecular biomarkers, such as post-translational modifications and autoantibodies, could be used in an attempt to improve disease detection and progression. In this thesis, I build on several iterations of the proximity ligation assay to develop and apply new adaptable methods to facilitate detection of proteins, autoantibodies and post-translational modifications. LÄS MER