Sökning: "carbohydrate-binding module"

Visar resultat 1 - 5 av 14 avhandlingar innehållade orden carbohydrate-binding module.

1. 1. Novel binding specificities engineered into the scaffold of a carbohydrate binding module

   Författare :Lavinia Cicortas Gunnarsson; Institutionen för immunteknologi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Bioteknik; Biotechnology; carbohydrate binding module; binding specificity; selection; combinatorial library; scaffold; protein engineering;

   Sammanfattning : The growing field of biotechnology is in constant need of proteins that can function as recognition tools for separational, analytical and therapeutic purposes. Different molecular engineering approaches are applied on natural proteins in order to create variants with desired properties. LÄS MER

3. 2. Functional studies and engineering of family 1 carbohydrate-binding modules

   Författare :Janne Lehtiö; KTH; []
   Nyckelord :Cellulose-binding; carbohydrate-binding modules; phage display; bacterial surface display; combinatorial protein library; protein engineering; Trichoderma reesei; Staphylococcus carnosus;

   Sammanfattning : The family 1 cellulose-binding modules (CBM1) form a groupof small, stable carbohydrate-binding proteins. These modulesare essential for fungal cellulosedegradation. This thesisdescribes both functional studies of the CBM1s as well asprotein engineering of the modules for several objectives. LÄS MER

4. 3. Engineering protein-carbohydrate interactions - lessons from natural and evolved carbohydrate binding modules

   Författare :Laura von Schantz; Institutionen för immunteknologi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; carbohydrate binding modules protein engineering affinity affinity maturation phage display specificity crystallography cellulose derivatives;

   Sammanfattning : Protein-carbohydrate interactions are of central role in all living organism. In the studies presented in this thesis several strategies for engineering such interactions have been investigated. In the first study the binding affinity of a carbohydrate binding module (CBM) was improved a 100-fold through affinity maturation. LÄS MER

5. 4. On the engineering of proteins: methods and applications for carbohydrate-active enzymes

   Författare :Fredrika Gullfot; Harry Brumer; Antoni Planas; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; enzyme engineering; rational design; directed evolution; DNA shuffling; glycosynthase; xyloglucan; xyloglucan endo-transglycosylase; retaining glycoside hydrolase; xyloglucanase; carbohydrate binding module; polysaccharide synthesis; Bioengineering; Bioteknik;

   Sammanfattning : This thesis presents the application of different protein engineering methods on enzymes and non-catalytic proteins that act upon xyloglucans. Xyloglucans are polysaccharides found as storage polymers in seeds and tubers, and as cross-linking glucans in the cell wall of plants. LÄS MER

7. 5. Understanding and manipulating primary cell walls in plant cell suspension cultures

   Författare :Felicia Leijon; Vincent Bulone; Katja Salomon Johansen; KTH; []
   Nyckelord :Callose synthase; carbohydrate-binding module; cell wall engineering; cellulose microfibril; cellulose nanocrystal; glycosyltransferase; mass spectrometry; plasmodesmata; Populus; primary cell wall; radiometric assay; spectrophotometric assay; Biotechnology; Bioteknologi;

   Sammanfattning : The cell wall is required for many aspects of plant function and development. It is also an accessible and renewable resource utilized both in unrefined forms and as raw material for further development. Increased knowledge regarding cell wall structure and components will contribute to better utilization of plants and the resources they provide. LÄS MER