Sökning: "SELEX"

Hittade 5 avhandlingar innehållade ordet SELEX.

  1. 1. Proximity Ligation : Transforming protein analysis into nucleic acid detection through proximity-dependent ligation of DNA sequence tagged protein-binders

    Författare :Simon Fredriksson; Larry Gold; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; Proximity ligation; proteomics; SELEX; antibody; DNA microarray; Molekylärmedicin; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Molecular Medicine; molekylär medicin genetik och patologi ;

    Sammanfattning : A novel technology for protein detection, proximity ligation, has been developed along with improved methods for in situ synthesis of DNA microarrays. Proximity ligation enables a specific and quantitative transformation of proteins present in a sample into nucleic acid sequences. LÄS MER

  3. 2. Determination of transcription factor binding specificities

    Författare :Arttu Jolma; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : The term "genetic code" refers to the way in which the information encoded in nucleic acids is converted into the amino-acid sequence of proteins. There is however also a second genetic code, one that is used by the cells to read the blueprints of the entire organism. LÄS MER

  4. 3. Proximity Ligation as a Universal Protein Detection Tool

    Författare :Mats Gullberg; Charles Cantor; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; Proximity ligation; proteomics; aptamer; antibody; molecular beacon; rolling circle amplification; Molekylärmedicin; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Molekylär medicin; Molecular Medicine;

    Sammanfattning : Among the great challenges in biology are the precise quantification of specific sets of proteins and analyses of their patterns of interaction on a much larger scale than is possible today.This thesis presents a novel protein detection technique - proximity ligation - and reports the development and application of a nucleic acid amplification technique, RCA. LÄS MER

  5. 4. Functionalised surfaces for bacterial discrimination

    Författare :Mohsen Golabi; Anthony Turner; Edwin Jager; Richard Luxton; Linköpings universitet; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biosensorer; Bakterier; Mönsterigenkänning;

    Sammanfattning : Bacterial detection and identification is a critical step in many arenas, including food and water safety, clinical diagnostics, bioprocess control and biosecurity. Social hygiene has a direct correlation with the strict control of microorganisms in these fields. LÄS MER

  7. 5. Deciphering the transcriptional regulation code in colorectal cancer genome

    Författare :Jian Yan; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Colorectal cancer (CRC) is the third most common cancer type to threaten life for both men and women in the developed world. The molecular mechanism of CRC is very complicated and involves changes in different categories of biological processes, among which a large number of mutations affecting either coding sequence of transcription factors (TFs) or their binding sites in genome are included. LÄS MER