Sökning: "recombinant protein expression"

Visar resultat 1 - 5 av 254 avhandlingar innehållade orden recombinant protein expression.

  1. 1. Recombinant subunit vaccines : protein immunogens

    Författare :Sissela Liljeqvist; KTH; []
    Nyckelord :;

    Sammanfattning : Recombinant techniques provide valuable tools for thedevelopment of modern subunit vaccines. In this thesisdifferent systems for production of recombinant subunitvaccines are presented. The strategies investigated includeprotein immunogens, live bacterial vectors and nucleicacids. LÄS MER

  3. 2. Recombinant amelogenin - Strategies for purification and protein processing

    Författare :Johan Svensson Bonde; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Recombinant amelogenin; protein purification; fusion protein; purification tag; enamel; hydroxyapatite; matrix protein; dental regeneration; wound healing; bone formation;

    Sammanfattning : Amelogenin is an extracellular matrix protein involved in formation of enamel during early tooth development. By interacting with the forming enamel crystallites amelogenin affects their growth, so that highly elongated hydroxyapatite crystals are formed. LÄS MER

  4. 3. Engineering yeast for improved recombinant protein production

    Författare :Yanyan Wang; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; point mutation; genome-scale modeling; recombinant protein; omics analysis; CRISPR Cas9; protein secretion; Saccharomyces cerevisiae;

    Sammanfattning : Recombinant proteins are broadly used from basic research to therapeutic development and include industrial enzymes and pharmaceutical proteins. The increasing demand for improved production and enhanced quality of recombinant proteins requires robust biotech-based strategies to overcome the limitations of protein extraction from natural sources. LÄS MER

  5. 4. Production and delivery of recombinant subunit vaccines

    Författare :Christin Andersson; KTH; []
    Nyckelord :Affibody; Affinity chromatography; Affinity purification; DNA immunization; Expression plasmid; Fusion protein; Hydrophobic tag; Iscoms; Lipid tagging; Malaria; Mammalian cell expression; Recombinant immunogen; Respiratory Syncytial Virus; Semliki Fo;

    Sammanfattning : Recombinant strategies are today dominating in thedevelopment of modern subunit vaccines. This thesis describesstrategies for the production and recovery of protein subunitimmunogens, and how genetic design of the expression vectorscan be used to adapt the immunogens for incorporation intoadjuvant systems. LÄS MER

  7. 5. Recombinant Enzymes in Pyrosequencing Technology

    Författare :Nader Nourizad; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; apyrase; pyrosequencing; pichia pastoris; protein expression; fusion protein; luciferase; DNA template; clone checking; DNA polymerase;

    Sammanfattning : Pyrosequencing is a DNA sequencing method based on thedetection of released pyrophosphate (PPi) during DNA synthesis.In a cascade of enzymatic reactions, visible light isgenerated, which is proportional to the number of nucleotidesincorporated into the DNA template. When dNTP(s) areincorporated into the DNA template, inorganic PPi is released. LÄS MER