Sökning: "periplasm"

Visar resultat 1 - 5 av 24 avhandlingar innehållade ordet periplasm.

  1. 1. Production and folding of proteins in the periplasm of Escherichia coli

    Författare :Rageia Elfageih; Jan-Willem de Gier; Hans-Georg Koch; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; periplasm; recombinant protein production; disulfide bond containing proteins; translation initiation region; protein translocation machinery; co-translational folding; neuraminidases; biokemi; Biochemistry;

    Sammanfattning : The Gram-negative bacterium E. coli is the most widely used host for the production of recombinant proteins. Disulfide bond containing recombinant proteins are usually produced in the periplasm of E. coli since in this compartment of the cell - in contrast to the cytoplasm - disulfide bond formation is promoted. LÄS MER

  3. 2. Nitric Oxide Reductase from Paracoccus denitrificans : A Proton Transfer Pathway from the “Wrong” Side

    Författare :Ulrika Flock; Pia Ädelroth; Simon de Vries; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; denitrification; nitric oxide reductase; heme copper oxidase superfamily; divergent member; proton transfer; electron transfer; single turnover; spectroscopy; periplasm; glutamate; proton pathway; Biochemistry; Biokemi; biokemi; Biochemistry;

    Sammanfattning : Denitrification is an anaerobic process performed by several soil bacteria as an alternative to aerobic respiration. A key-step in denitrification (the N-N-bond is made) is catalyzed by nitric oxide reductase (NOR); 2NO + 2e- + 2H+ → N2O + H2O. LÄS MER

  4. 3. Membrane and secretory protein production in Escherichia coli

    Författare :Alexandros Karyolaimos; Jan-Willem de Gier; Matthew DeLisa; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; recombinant protein; tunable protein production; cytoplasmic membrane; periplasm; signal peptide; force profile analysis; co-translational folding; Biochemistry; biokemi;

    Sammanfattning : Escherichia coli (E. coli) is the most widely used bacterium for the production of recombinant proteins. However, the production of proteins in its cytoplasmic membrane or periplasm is challenging. Therefore, the aim of this doctoral thesis was to develop setups for enhancing the production of membrane and secretory proteins in E. LÄS MER

  5. 4. The Structural Modularity and Inherent Dynamics of the DegP Protease together with its Intertwined Role in the Bacterial Periplasm

    Författare :Darios Šulskis; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; serine protease; DegP; nuclear magnetic resonance; E. coli; protein quality control; chaperone; HtrA;

    Sammanfattning : The protein quality control machinery is a delicate and integrated network of molecular tools working together to fold or remove unwanted proteins from the cell. A distinct set of “gatekeepers” are involved in this process including molecular chaperones, proteases, oxidoreductases, transferases, and many others in both eukaryotic and prokaryotic organisms. LÄS MER

  7. 5. Studies of pore-forming bacterial protein toxins in Escherichia coli

    Författare :Constance Enow; Bernt Eric Uhlin; Sun Nyunt Wai; Mikael Rehn; Umeå universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; molekylärbiologi; Molecular Biology;

    Sammanfattning : Escherichia coli, a Gram-negative bacterium, which can be classified into three groups: the commensal, intestinal pathogenic (IPEC) and extra-intestinal pathogenic (ExPEC) E. coli. The cytolysin A (ClyA) protein, a 34-kDa pore-forming toxin, encoded by a gene found in both non-pathogenic and pathogenic E. LÄS MER