Sökning: "non-viral vector"

Visar resultat 1 - 5 av 7 avhandlingar innehållade orden non-viral vector.

  1. 1. Dendrimer-mediated gene delivery: Effect of polymer structure on cellular response

    Författare :Kristina Fant; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; gene therapy; DNA condensation; cationic polymers; toxicity; non-viral vector; drug delivery; polyplex; PAMAM dendrimers;

    Sammanfattning : The delivery of nucleic acids to cells, in vitro and in vivo, is becoming a realistic approach for intervention with gene expression at a molecular level. It can be used to repair malfunctioning genes or to introduce new functionalities, with the most important application being clinical treatment of human diseases. LÄS MER

  3. 2. Condensation of DNA for Gene Delivery: Studies on Vector-Nucleic Acid Interactions and Transfection Efficiency

    Författare :Kristina Fant; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; dendrimer; endocytosis; uptake; gene delivery; optical spectroscopy; structure-activity relationship; DNA; cell-penetrating peptide;

    Sammanfattning : This thesis concerns the delivery of nucleic acids into cells and tissues with the aim to change or correct gene expression. The field of gene delivery has the ultimate goal of producing genetic drugs for clinical applications, and the activity in this area is intense although so far the use is limited to research purposes. LÄS MER

  4. 3. Cell-penetrating peptides as delivery vectors for oligonucleotides and proteins : Studies on applications and toxicity

    Författare :Peter Järver; Ülo Langel; Gilles Divita; Stockholms universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Neurochemistry; Neurokemi; neurokemi med molekylär neurobiologi; Neurochemistry with Molecular Neurobiology;

    Sammanfattning : Cell-penetrating peptides (CPPs) have for a little bit more than a decade been employed as delivery vectors for a wide range of cargoes, ranging from gold particles to entire plasmids. Although CPP are well studied and utilized in numerous publications, our knowledge about CPP mediated transport is still poor. LÄS MER

  5. 4. Nucleic acid delivery : reports from the search of the magic bullet

    Författare :Joana Viola; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Gene therapy is regarded as one of the most promising therapeutic approaches, since it has the potential to treat disorders by correcting malformations at the nucleic acids level rather than proteins, as opposed to conventional medicine. However, for non-viral gene therapy to successfully fulfill the requirements associated to the “magic bullet” has proven difficult. LÄS MER

  7. 5. Development of novel DNA vaccine and immunotherapeutic approaches

    Författare :Maroof Hasan; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Many potential DNA vaccination and immunotherapeutic strategies has been explored in both animal models and human settings in recent years. However, the failure of efficient and safe method development suggests that it is time to rethink, since more innovative approaches are needed for better control of infection and immunotherapy. LÄS MER