Sökning: "fusion tags"

Visar resultat 1 - 5 av 17 avhandlingar innehållade orden fusion tags.

1. 1. Effects of fusion tags on protein partitioning In aqueous two-phase systems and use in primary protein recovery

   Författare :Cynthia Hassinen; KTH; []
   Nyckelord :E. coli; Aqueous two-phase systems; Fusion proteins; Hydrophobic interaction chromatography; Expanded bed adsorption; Beta-galactosidase; Klenow polymerase; Z-domain; Peptide tags;

   Sammanfattning : The two techniques aqueoustwo-phase partitioning and expanded bed adsorption that bothare suitable for primary protein recovery were studied. Most ofthe work was focused on partition in aqueous two-phase systemsand in particular on the possibility to effect the partitionbehaviour by fusion of short peptide tags or protein domains tothe target protein. LÄS MER

3. 2. Metal Binding Tags - Characterisation, Use in Bioseparation and Applications of Green Fluorescent Fusion Proteins

   Författare :Florent Bernaudat; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Proteiner; enzymologi; enzymology; E. coli; Immobilised metal ion affinity chromatography; Affinity tags; Green fluorescent protein; Surface plasmon resonance; Genetic engineering; Screening; Proteins; Immunoassay; Biotechnology; Bioteknik;

   Sammanfattning : Most of the recombinant proteins produced nowadays are fused to affinity tags, in order to facilitate their purification through affinity chromato-graphy. Out the different tags available, poly-histidine tags are among the most commonly used and can help purification through immobilised metal ion affinity chromatography (IMAC). LÄS MER

4. 3. Hydrophobic Fusion Tags: Implications For Bioseparation and Cellular Expression

   Författare :Anna Collén; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; protein production; secretion; aqueous two-phase systems; Trichoderma reesei; endoglucanase; Biochemistry; Metabolism; Biokemi; metabolism;

   Sammanfattning : The studies in this thesis have shown that the partitioning of endoglucanase I (EGI, Cel7B) from T. reesei could be significantly improved by relatively minor genetic engineering. By adding short peptides composed of tryptophan and proline residues to EGI, extreme partitioning could be obtained. LÄS MER

5. 4. Protein production, characterization and structure determination in structural genomics

   Författare :Esmeralda A. Woestenenk; Torleif Härd; Göran Karlsson; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biology; protein production; protein solubility; fusion tags; nuclear magnetic resonance; structure determination; Biologi; Biology; Biologi;

   Sammanfattning : This thesis covers the process from expression of a heterologous gene in Escherichia coli to structure determination of a protein by nuclear magnetic resonance (NMR) spectroscopy. The first part concerns structural genomics-related parallel screening studies on the effect of fusion tags (in particular the His tag) on protein solubility and the use of fusion tags in fast, parallel purification protocols intended for initial biophysical characterization of human proteins produced in E. LÄS MER

7. 5. HYDROPHOBIC PEPTIDE TAGS - As tools in bioseparation and investigation of recombinant proteins

   Författare :Sara Fexby; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; E. coli; Biochemical technology; Biokemisk teknik; fusion protein; tyrosine; hydrophobicity; Hydrophobic interaction chromatography; aqueous two-phase system;

   Sammanfattning : This thesis describes the possibility of using hydrophobic peptide tags as tools for bioseparation and for the investigation of recombinant proteins. The hydrophobicity of a protein can easily be changed by minor modifications, such as fusions of tyrosine-containing peptides. LÄS MER