Sökning: "complementation assay"

Visar resultat 1 - 5 av 9 avhandlingar innehållade orden complementation assay.

1. 1. Novel approaches to mucosal vaccine development Strategies in vaccine antigen production, construction of a novel mucosal adjuvant and studies of its mode of action

   Författare :Manuela Terrinoni; Göteborgs universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; vaccine development; plasmid maintenance; Gram-negative bacteria; ; essential genes; complementation; Cholera Toxin; adjuvanticity; NF-kB; mmCT; Gram-negative bacteria;

   Sammanfattning : Although most infections begin at a mucosal surface and may be prevented by effective vaccine stimulation of the local mucosal immune system, there are so far only a few mucosal vaccines available for human use. This thesis spans several areas that are important for future development of mucosal vaccines. LÄS MER

3. 2. Engineering Yeast for Functional Analysis of Human Cu Transport Proteins

   Författare :Kumaravel Ponnandai Schanmugavel; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Cu transport; yeast model; Atox1; genetic engineering; Wilson disease protein; ATP7B; complementation assay; yeast growth; metal-binding domain; mechanisms.;

   Sammanfattning : ABSTRACT       Copper (Cu) is an important trace element that plays a vital role in several biological processes. Many proteins that are involved in various biochemical pathways require Cu as a cofactor. LÄS MER

4. 3. On bacterial formats in protein library technology

   Författare :Per-Åke Löfdahl; Per-Åke Nygren; Mikael Widersten; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Affibody molecule; β-lactamase; combinatorial library; green fluorescent protein GFP ; protein engineering; protein fragment complementation assay PCA ; selection; TEVprotease; TNF-α; Bioengineering; Bioteknik; Enzyme engineering; Enzymteknik;

   Sammanfattning : Millions of years of evolution have resulted in an immense number of different proteins, which participate in virtually every process within cells and thus are of utmost importance for allknown forms of life. In addition, there are several examples of natural proteins which have found use in applications outside their natural environment, such as the use of enzymes infood industry and washing powders or the use of antibodies in diagnostic, bioseparation or therapeutic applications. LÄS MER

5. 4. A screen for mutations affecting PNS development in Drosophila identifies the trim gene, dappled

   Författare :Fergal O'Farrell; Per Kylsten; Jan-Åke Gustafsson; Stefan Thor; Södertörns högskola; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

   Sammanfattning : The peripheral nervous system of Drosophila melanogaster contains a variety of sense organs, ranging from the relatively simple four celled bristle organ to the more complex compound eye. The development of each organ type is well described, providing a useful backdrop for functional studies of genes acting in one or more of the many processes involved in organogenesis. LÄS MER

7. 5. Myc oncoprotein cofactor interactions : function, regulation and targeting

   Författare :Karin Ridderstråle; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : The members of the Myc-family of proto-oncoproteins c-Myc, N-Myc and L-Myc are transcription factors exerting their effect via protein-protein interactions. The Myc proteins are basic-helix-loop-helix-leucine-zipper (bHLHzip) transcription factors, which after heterodimerization with their obligatory partner Max, bind to E-boxes in target gene promoters and regulate the expression of 10-15% of all genes by the recruitment of different cofactors. LÄS MER