Sökning: "Nucleic Acid genetics"

Visar resultat 1 - 5 av 10 avhandlingar innehållade orden Nucleic Acid genetics.

  1. 1. Mitotic recombination in mammalian cells

    Författare :Catherine Arnaudeau; Harry Vrieling; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; toxikologisk genetik; Genetic Toxicology;

    Sammanfattning : Recombination is usually defined as the exchange of genetic material between two strands or regions of nucleic acids. This process occurs in all known organisms and is highly conserved, especially among higher eukaryotes. Various types of recombination, involving homologous or non-homologous nucleic acid sequences, are known to exist. LÄS MER

  3. 2. Molecular Tools for Nucleic Acid Analysis

    Författare :Deirdre O'Meara; KTH; []
    Nyckelord :nucleic acid capture; modular probes; biosensor; bio-magnetic separation; hepatitis C; sequencing; pyrosequencing; mutation detection; HIV-1; drug resistance; SNP; allele-specific extension; apyrase; genotyping.;

    Sammanfattning : Nucleic acid technology has assumed an essential role invarious areas ofin vitrodiagnostics ranging from infectious diseasediagnosis to human genetics. An important requirement of suchmolecular methods is that they achieve high sensitivity andspecificity with a fast turnaround time in a cost-effectivemanner. LÄS MER

  4. 3. Proximity Ligation : Transforming protein analysis into nucleic acid detection through proximity-dependent ligation of DNA sequence tagged protein-binders

    Författare :Simon Fredriksson; Larry Gold; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; Proximity ligation; proteomics; SELEX; antibody; DNA microarray; Molekylärmedicin; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Molecular Medicine; molekylär medicin genetik och patologi ;

    Sammanfattning : A novel technology for protein detection, proximity ligation, has been developed along with improved methods for in situ synthesis of DNA microarrays. Proximity ligation enables a specific and quantitative transformation of proteins present in a sample into nucleic acid sequences. LÄS MER

  5. 4. Genetic Analyses using Rolling Circle or PCR Amplified Padlock Probes

    Författare :Johan Banér; Sanjay Tyagi; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; Padlock probes; rolling circle amplification; PCR; genotyping; microarray; expression analysis; Molekylärmedicin; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Medical Genetics; medicinsk genetik;

    Sammanfattning : Padlock probes are useful in a variety of genetic applications, some of which require that the probes are amplified in order to generate detectable signals. Two general padlock amplification methods, RCA and PCR, are discussed in this thesis. LÄS MER

  7. 5. From Tissue to Mutations : Genetic Profiling of Colorectal Cancer

    Författare :Lucy Mathot; Tobias Sjöblom; Mats Nilsson; Alberto Bardelli; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Nucleic acid extraction; genotyping; targeted sequencing; mutation analysis; colorectal cancer; metastatic disease; Medical Science; Medicinsk vetenskap;

    Sammanfattning : Comprehensive characterisation of the mutational landscapes of solid tumours is a multistep process involving the collection of suitable samples, the extraction of nucleic acids and the preparation of these materials for mutational analyses. In this thesis, I aimed to develop a streamlined process for the analysis of colorectal cancer (CRC) patient samples in order to identify novel mutations that hallmark the development of advanced disease. LÄS MER