Sökning: "Live-cell microscopy"

Visar resultat 1 - 5 av 20 avhandlingar innehållade orden Live-cell microscopy.

1. 1. Automating STED microscopy for functional and structural live-cell imaging

   Författare :Jonatan Alvelid; Ilaria Testa; Eggeling Christian; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; STED; microscopy; nanoscopy; super-resolution microscopy; automation; image analysis; fluorophores; Biologisk fysik; Biological Physics;

   Sammanfattning : Optical microscopy imaging methods are today invaluable tools for studies in life sciences as they allow visualization of biological systems, tissues, cells, and sub-cellular compartments from millimetres down to nanometres. The invention and development of nanoscopy in the past 20 years has pushed fluorescence microscopy down to the nanoscale, reaching beyond the natural diffraction limit of light that does not allow focusing of visible light below sizes of around 200 nm, and into the realm of what was previously only thought possible with electron microscopy. LÄS MER

3. 2. Development of quantitative optical microscopy methods for single cell analysis

   Författare :Katarina Logg; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; protein localization; quantitative optical microscopy; live cell imaging; image analysis; cellular stress;

   Sammanfattning : Optical microscopy has brought powerful methods to biological research, in particular the possibility to assay dynamic molecular processes within living individual cells. This thesis deals with various aspects of fluorescence and Raman microscopy, such as the possibility of avoiding cellular damage or stress caused by illumination, how to manipulate single cells for spectroscopic analysis and how to quantify images automatically. LÄS MER

4. 3. Mechanisms regulating Natural Killer Cell Cytotoxicity

   Författare :Hanna van Ooijen; Björn Önfelt; Karl-Johan Malmberg; Karolin Guldevall; Morgan Huse; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Natural Killer cell; Live-cell microscopy; Single cell; 3D; Microwell;

   Sammanfattning : Over the last 50 years, cancer survival rates have steadily improved thanks to earlier detection and novel treatment regimens. For example, drugs that act on the immune system, so-called immunotherapy, have drastically increased the prospect of survival for patients suffering from some of the most aggressive cancer types including advanced malignant melanoma. LÄS MER

5. 4. Detection and quantification of lipid-mediated siRNA delivery to the cytosol

   Författare :Hampus Hedlund; Tumörmikromiljö; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; RNA Interference; RNA Therapeutics; Endosomal Escape; Lipid Mediated Delivery; Live-Cell Imaging;

   Sammanfattning : Cancer is one of the leading causes of death globally. Novel therapeutics is urgently needed to advance treatment, especially for generalized disease where current treatment options have a poor prognosis. In particular, targeted therapy that can address genetic changes would be of great value. LÄS MER

7. 5. Advancing DNA-based proximity methods

   Författare :Mattias Leino; Ola Söderberg; John Löfblom; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; in situ; proximity dependent intiation of hybridization chain reaction; hybridization chain reaction; Oligonucleotide design; proximity ligation assay; protein interaction; microscopy; purification; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

   Sammanfattning : Cellular functions are governed by intricate chains of interactions between proteins. In order to properly understand cellular biology one must look into, not only protein function, but also its interacting network. LÄS MER