Sökning: "Amplified single molecule"

Visar resultat 1 - 5 av 16 avhandlingar innehållade orden Amplified single molecule.

1. 1. Detection and Sequencing of Amplified Single Molecules

   Författare :Rongqin Ke; Mats Nilsson; Ulf Landegren; Radoje Drmanac; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; padlock probes; proximity ligation assay; rolling circle amplification; circle-to-circle amplification; single molecule detection; amplified single molecules; sequencing; in situ; single cell; Molecular Biotechnology; Molekylär bioteknik;

   Sammanfattning : Improved analytical methods provide new opportunities for both biological research and medical applications. This thesis describes several novel molecular techniques for nucleic acid and protein analysis based on detection or sequencing of amplified single molecules (ASMs). LÄS MER

3. 2. Biomolecular Analysis by Dual-Tag Microarrays and Single Molecule Amplification

   Författare :Olle Ericsson; Ulf Landegren; Mats Nilsson; Edwin Southern; Uppsala universitet; []
   Nyckelord :Molecular medicine; Microarray; proximity ligation; Padlock probe; Rolling circle amplification; Splicing; single molecule; Molekylärmedicin;

   Sammanfattning : Padlock probes and proximity ligation are two powerful molecular tools for detection of nucleic acids and proteins, respectively. Both methods result in the formation of DNA reporter molecules upon recognition of specific target molecules. LÄS MER

4. 3. Single-Molecule Detection and Optical Scanning in Miniaturized Formats

   Författare :Jonas Melin; Fredrik Nikolajeff; Mats Nilsson; Hugh Fan; Uppsala universitet; []
   Nyckelord :Biotechnology; microfluidics; single-molecule detection; MOEMS; μTAS; lab-on-a-chip; RCA; thermoplastics; injection molding; hot embossing; Bioteknik;

   Sammanfattning : In later years polymer replication techniques have become a frequently employed fabrication method for microfluidic and micro-optical devices. This thesis describes applications and further developments of microstructures replicated in polymer materials. LÄS MER

5. 4. Development of Electrical Readouts for Amplified Single Molecule Detection

   Författare :Camilla Russell; Mats Nilsson; Björn Högberg; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Rolling circle amplification; metallization; electrical DNA detection; magnetic nanoparticles; dielectrophoresis; boranephosphonate modified nucleotides; DNA elongation; Biokemi; Biochemistry;

   Sammanfattning : Molecular diagnostics is a fast growing field with new technologies being developed constantly. There is a demand for more sophisticated molecular tools able to detect a multitude of molecules on a single molecule level with high specificity, able to distinguish them from other similar molecules. LÄS MER

7. 5. Readout Strategies for Biomolecular Analyses

   Författare :Jenny Göransson; Mats Nilsson; Charles Cantor; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Padlock probe; Selector probe; Rolling circle amplification; Single molecule detection; Amplified single molecule; Random array; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ;

   Sammanfattning : This thesis describes three readout formats for molecular analyses. A common feature in all works is probing techniques that upon specific target recognition ideally results in equimolar amounts of DNA circles. These are then specifically amplified and detected using any of the techniques presented herein. LÄS MER