Sökning: "preparative liquid chromatography"

Visar resultat 1 - 5 av 16 avhandlingar innehållade orden preparative liquid chromatography.

  1. 1. Adsorption Studies with Liquid Chromatography : Experimental Preparations for Thorough Determination of Adsorption Data

    Författare :Lena Edström; Jonas Bergquist; Lars Hagel; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Liquid chromatography; HPLC; UHPLC; Reversed phase; Preparative chromatography; Adsorption isotherm; Injection profile; Sample pH; pH stable conditions; Peak deformation; Band distortion; Overloaded band; Chiral preparative chromatography; Analytical Chemistry; Analytisk kemi;

    Sammanfattning : Analytical chemistry is a field with a vast variety of applications. A robust companion in the field is liquid chromatography, the method used in this thesis, which is an established workhorse and a versatile tool in many different disciplines. LÄS MER

  3. 2. Adsorption Isotherm Parameter Estimation in Nonlinear Liquid Chromatography

    Författare :Patrik Forssén; Bertil Gustafsson; Torgny Fornstedt; Georges Guiochon; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; High performance liquid chromatography HPLC ; Preparative chromatography; Competitive adsorption isotherm parameters; Perturbation peak PP method; Inverse method IM ; Computer simulation; Parameter estimation; Numerical method; Scientific Computing; Beräkningsvetenskap;

    Sammanfattning : This thesis concerns the development and validation of methods for the industrially important area of adsorption isotherm parameter estimation in preparative, nonlinear high performance liquid chromatography (HPLC). Preparative chromatography is a powerful separation method to get pure compounds from more or less complex liquid mixtures, e.g. LÄS MER

  4. 3. Development and improvement of methods for characterization of HPLC stationary phases

    Författare :Torgny Undin; Torgny Fornstedt; Jörgen Samuelsson; Anders Törncrona; Johan Ekeroth; Douglas Westerlund; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Adsorption isotherms; Elution by characteristic points; Inflection points; Single component adsorption; Elution by characteristic points method; Slope data; ECP-slope method; Adsorption; Adsorption energy distribution; Adsorption isotherms; AED; Characterization of adsorption processes; Chromatographic analysis; Chromatography; Hydrophobic-subtraction method HSM ; Hydrophobicity; Linear methods; Linear Models; Liquid chromatography; metoprolol; Non-linear methods; Nonlinear methods; phenol; priority journal; propranolol; Retention mechanism; reversed phase liquid chromatography; Solvation; Subtraction method; Thermodynamics; Chemistry with specialization in Surface Biotechnology; Kemi med inriktning mot ytbioteknik;

    Sammanfattning : High Performance Liquid Chromatography (HPLC) is a widely used tech-nique both for detecting and purifying substances in academy and in the industry. In order to facilitate the use of, and knowledge in HPLC, character-ization of stationary phases is of utmost importance. LÄS MER

  5. 4. Development of Methods for Phase System Characterization in Liquid Chromatography

    Författare :Jörgen Samuelsson; Torgny Fornstedt; Karin Caldwell; Attila Felinger; Uppsala universitet; []
    Nyckelord :Analytical chemistry; Liquid chromatography; Adsorption isotherms; Adsorption energy distribution; Tracer pulse method; Elution by characteristic points; Peak deformation; Validation; Hybrid column material; Alkaline separation; Injection-profiles; Analytisk kemi;

    Sammanfattning : The aim of this thesis is first and foremost to improve the fundamental knowledge of nonlinear and preparative separation theory by focusing on some of the remaining “white spots” on the theoretical chromatographic map. Secondly, the acquired knowledge is used to develop, validate and execute new methods for phase characterization in liquid chromatography. LÄS MER

  7. 5. Development and Validation of HPLC Methods for Analytical and Preparative Purposes

    Författare :Johan Lindholm; Torgny Fornstedt; Karin Caldwell; Karl-Gustaf Wahlund; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Chemistry; High performance liquid chromatography HPLC ; Analytical biotechnology; Sample preparation; Diode-array-detection DAD ; Mass spectrometry MS ; Validation; Preparative chromatography; Competitive isotherm parameters; Perturbation peak PP method; Kemi; Chemistry; Kemi;

    Sammanfattning : This thesis concerns the development and validation of high performance liquid chromatography (HPLC) methods aimed for two industrially important areas: (i) analysis of biotechnological synthesis and (ii) determination of adsorption isotherm parameters. There is today a lack of detailed recommendations for analytical procedures in the field of biotechnological production of drugs. LÄS MER