Sökning: "Proximity ligation assay"

Visar resultat 21 - 25 av 57 avhandlingar innehållade orden Proximity ligation assay.

1. 21. Visualizing Interacting Biomolecules In Situ

   Författare :Irene Weibrecht; Ola Söderberg; Ulf Landegren; Banafshe Larijani; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proximity ligation; in situ PLA; padlock probe; rolling circle amplification; flow cytometry; in situ; single cell; single molecule; protein interaction; protein-DNA interaction; posttranslational modification; Molecular medicine; Molekylär medicin; Molekylär medicin; Molecular Medicine;

   Sammanfattning : Intra- and intercellular information is communicated by posttranslational modifications (PTMs) and protein-protein interactions, transducing information over cell membranes and to the nucleus. A cells capability to respond to stimuli by several highly complex and dynamic signaling networks provides the basis for rapid responses and is fundamental for the cellular collaborations required in a multicellular organism. LÄS MER

3. 22. DNA-Mediated Detection and Profiling of Protein Complexes

   Författare :Maria Hammond; Ulf Landegren; Masood Kamali-Moghaddam; Christof Niemeyer; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Proximity ligation assay; Protein complexes; Protein interactions; Biomarkers; Prions; Antibodies; Medicinsk vetenskap; Medical Science;

   Sammanfattning : Proteins are the effector molecules of life. They are encoded in DNA that is inherited from generation to generation, but most cellular functions are executed by proteins. Proteins rarely act on their own – most actions are carried out through an interplay of tens of proteins and other biomolecules. LÄS MER

4. 23. Analysis of signaling pathway activity in single cells using the in situ Proximity Ligation Assay

   Författare :Linda Arngården; Ola Söderberg; Niclas Karlsson; Uppsala universitet; []
   Nyckelord :cell signaling; Wnt; Hippo; TGFB; Molekylär medicin; Molecular Medicine;

   Sammanfattning : A cell that senses signals from its environment uses proteins for signal transduction via post translational modifications (PTMs) and protein- protein interactions (PPIs) from cell membrane into the nucleus where genes controlling cell proliferation, differentiation and apoptosis can be turned on or off, i.e. LÄS MER

5. 24. Ex‘PLA’ining the progression of pathological proteins in Alzheimer’s and Parkinson’s diseases : see(d)ing is believing

   Författare :Anish Behere; Joakim Bergström; Sara Ekmark-Lewén; Martin Ingelsson; Stina Syvänen; Hilal Lashuel; Uppsala universitet; []
   Nyckelord :Alpha synuclein; Tau; Proteoforms; Phosphorylation; Oligomers; Proximity Ligation Assay; sensitive detection of pathology; Alzheimer s disease; Parkinson s disease; pS129; pS202 pT205; pT231; PFFs;

   Sammanfattning : Alzheimer’s disease (AD) and Parkinson’s disease (PD) are the two most common forms of neurodegenerative disorders affecting approximately 50 million people worldwide. The underlying neuropathological processes leading to AD and PD share many similarities, i.e. aberrant protein aggregation of tau and alpha-synuclein (αSyn) in the brain. LÄS MER

7. 25. Pathophysiological effects of alpha-synuclein on SNARE complex proteins in models of alpha-synucleinopathies

   Författare :Emma Brolin; Joakim Bergström; Anna Erlandsson; Martin Ingelsson; Arianna Bellucci; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Alpha-synuclein; Parkinson’s disease; synaptosome; SNARE complex; proximity ligation assay; extracellular vesicles;

   Sammanfattning : Accumulation and spread of alpha-synuclein (α-syn) aggregates are central to the disease pathogenesis of Parkinson’s disease and dementia with Lewy bodies, collectively known as α-synucleinopathies. Native α-syn is a monomeric presynaptic protein that can act as a molecular chaperone for the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex assembly. LÄS MER