Sökning: "xyloglucan endo-transglycosylase"

Visar resultat 1 - 5 av 7 avhandlingar innehållade orden xyloglucan endo-transglycosylase.

  1. 1. Xyloglucan-active enzymes : properties, structures and applications

    Författare :Martin J. Baumann; Tuula T. Teeri; Harry Brumer; Vincent Eijsink; KTH; []
    Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; xyloglucan; xyloglucan endo-transglycosylase hydrolase; xyloglucan endo-hydrolase; xyloglucan endo-transglycosylase; XET; xyloglucan oligosaccharides; synthesis; carbohydrate; cellulose; crystal structure; fiber; surface modification; Populus tremula x Populus tremuloides; Hybrid aspen; nasturtium; NXG; Biochemistry; Biokemi;

    Sammanfattning : Cellulosabaserade material är världens rikligast förekommande förnyelsebara råvara. Växters cellväggar är naturliga kompositmaterial där den kristallina cellulosan är inbäddad i en väv av hemicellulosa, strukturproteiner och lignin. LÄS MER

  2. 2. Production and engineering of a xyloglucan endo-transglycosylase from Populus tremula x tremuloides

    Författare :Maria Henriksson; Tuula Teeri; KTH; []
    Nyckelord :AGRICULTURAL SCIENCES; LANTBRUKSVETENSKAPER; LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES; xyloglucan endo-transglycosylase; retaining glycoside hydrolase; glycosynthase; Pichia pastoris; fed-batch fermentation; expanded-bed adsorption; Plant biotechnology; Växtbioteknik;

    Sammanfattning : The aim of this work was to develop a production process for the enzyme xyloglucan endo-transglycosylase from Populus tremula x tremuloides (PttXET16-34). The natural transglycosylating activity of this enzyme has previously been employed in a XET-Technology. LÄS MER

  3. 3. Synthesis of xyloglucan oligo- and polysaccharides with glycosynthase technology

    Författare :Fredrika Gullfot; Harry Brumer; Tuula Teeri; Jerry Ståhlberg; Mats Martinelle; KTH; []
    Nyckelord :ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; glycosynthase; xyloglucan; xyloglucan endo-transglycosylase; retaining glycoside hydrolase; xyloglucanase; polysaccharide synthesis; Bioengineering; Bioteknik; Enzyme engineering; Enzymteknik; Bioorganical synthesis; Bioorganisk syntes; Biomaterials; Biomaterial;

    Sammanfattning : Xyloglucans are polysaccharides found as storage polymers in seeds and tubers, and as cross-linking glycans in the cell wall of plants. Their structure is complex with intricate branching patterns, which contribute to the physical properties of the polysaccharide including its binding to and interaction with other glycans such as cellulose. LÄS MER

  4. 4. On the engineering of proteins: methods and applications for carbohydrate-active enzymes

    Författare :Fredrika Gullfot; Harry Brumer; Antoni Planas; KTH; []
    Nyckelord :ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; enzyme engineering; rational design; directed evolution; DNA shuffling; glycosynthase; xyloglucan; xyloglucan endo-transglycosylase; retaining glycoside hydrolase; xyloglucanase; carbohydrate binding module; polysaccharide synthesis; Bioengineering; Bioteknik;

    Sammanfattning : This thesis presents the application of different protein engineering methods on enzymes and non-catalytic proteins that act upon xyloglucans. Xyloglucans are polysaccharides found as storage polymers in seeds and tubers, and as cross-linking glucans in the cell wall of plants. LÄS MER

  5. 5. In vitro and in vivo approaches in the characterization of XTH gene products

    Författare :Nomchit Kaewthai; Harry Brumer; Jocelyn Rose; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Arabiodopsis thaliana; Hordeum vulgare; plant cell wall; xyloglucan; glycoside hydrolase family 16; xyloglucan endo-transglycosylase hydrolase gene family; xyloglucan endo-hydrolase; heterologous protein expression; Pichia pastoris; T-DNA insertion; in situ XET XEH assay; high-througput screening; Plant physiology; Växtfysiologi; Biochemistry; Biokemi; Functional genomics; Funktionsgenomik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

    Sammanfattning : ABSTRACT The xyloglucan endo-transglycosylase/hydrolase (XTH) genes are found in all vascular and some nonvascular plants. The XTH genes encode proteins which comprise a subfamily of glycoside hydrolase (GH) family 16 in the Carbohydrate-Active enZYmes (CAZY) classification. LÄS MER