Sökning: "xylitol dehydrogenase"

Visar resultat 1 - 5 av 12 avhandlingar innehållade orden xylitol dehydrogenase.

  1. 1. Redox balancing in recombinant strains of Saccharomyces cerevisiae

    Författare :Mikael Anderlund; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; xylose isomerase; xylitol dehydrogenase; xylose reductase; glycerol; xylitol; xylose; E. coli; A. vinelandii; T. thermophilus; S. cerevisiae; P. stipitis; transhydrogenase.; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Sammanfattning : In metabolically engineered Saccharomyces cerevisiae expressing Pichia stipitis XYL1 and XYL2 genes, encoding xylose reductase (XR) and xylitol dehydrogenase (XDH), respectively, xylitol is excreted as the major product during anaerobic xylose fermentation and only low yields of ethanol are produced. This has been interpreted as a result of the dual cofactor dependence of XR and the exclusive use of NAD+ by XDH. LÄS MER

  3. 2. Trametes versicolor as biodegrader and biocatalyst when using lignocellulose for ethanol production

    Författare :Marie Holmgren; Anita Sellstedt; Mohammad Taherzadeh; Umeå universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Lignocellulose; 5-carbon; 6-carbon; Trametes versicolor; xylose reductase; xylitol dehydrogenase; xylulokinase; phenolics; levulinic acid; HMF; furfural; biodegrader; biocatalyst; pretreatment; xylanase; beta-glucosidase;

    Sammanfattning : Energy consumption has increased rapidly during the last century due to population growth and greater industrialization. Lignocellulosic-based biofuels are being developed as alternatives to fossil fuels. For many years the question of how 5-carbon sugars in biomass are utilized in nature has been a vexed one. LÄS MER

  4. 3. Metabolic Engineering of Xylose-Utilising Saccharomyces cerevisiae Strains. A Closer Look at Recombinant Strains Based on the Xylose Reductase-Xylitol Dehydrogenase Pathway

    Författare :Marie Jeppsson; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi; microarrays; cofactors; XK; XDH; XR; lignocellulose; xylose; ethanol; Saccharomyces cerevisiae; yeast;

    Sammanfattning : Saccharomyces cerevisiae produces ethanol efficiently from the hexose sugars in lignocellulose hydrolysates, but it can not utilise pentose sugars such as xylose and arabinose. Stable xylose-utilising S. LÄS MER

  5. 4. Metabolic engineering and random mutagenesis for improved xylose utilisation of Saccharomyces cerevisiae

    Författare :Fredrik Wahlbom; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biokemisk teknik; Biochemical technology; mykologi; virologi; bacteriology; Microbiology; Kemiteknik och kemisk teknologi; Chemical technology and engineering; ethanol; Saccharomyces; xylose; virology; mycology; Mikrobiologi; bakteriologi;

    Sammanfattning : In this thesis I have summarised my work on the analysis and improvement of xylose utilisation by recombinant S. cerevisiae. A metabolic flux model was developed and used to analyse the intracellular fluxes in the recombinant xylose utilising S. cerevisiae TMB 3001 cultivated in chemostat at various dilution rates and xylose/glucose concentrations. LÄS MER

  7. 5. Engineering xylose and arabinose metabolism in recombinant Saccharomyces cerevisiae

    Författare :Kaisa Karhumaa; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; virologi; bakteriologi; mycology; Mikrobiologi; bacteriology; Saccharomyces cerevisiae ; arabinose; xylose; fermentation; lignocellulose; yeast; mykologi; Biochemical technology; Biokemisk teknik; virology; Microbiology; ethanol;

    Sammanfattning : Utilization of all sugars in lignocellulose hydrolysates is a prerequisite for economically feasible bioethanol production. The yeast commonly used for industrial ethanol production, Saccharomyces cerevisiae, is naturally unable to utilize pentose sugars xylose and arabinose, which constitute a large fraction of many lignocellulosic materials. LÄS MER