Sökning: "whole-cell"

Visar resultat 1 - 5 av 107 avhandlingar innehållade ordet whole-cell.

1. 1. New Roads for an Ancient Enzyme : Whole-Cell Studies and New Cofactors for [FeFe] Hydrogenases

   Författare :Marco Lorenzi; Gustav Berggren; Hannah Shafaat; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; [FeFe] hydrogenases; hydrogen; catalysis; photocatalysis; spectroscopy; whole-cell; semi-artificial enzymes; Chemistry with specialization in Molecular Biomimetics; Kemi med inriktning mot molekylär biomimetik;

   Sammanfattning : [FeFe] hydrogenases rare Nature’s best H2-processing catalysts, and one of the best candidates to satisfy societal need for cheap and efficient catalyst for H2-evolution. These enzymes owe their remarkable catalytic activities to their organometallic active site, called “H-cluster”. LÄS MER

3. 2. Surface expression using the AIDA autotransporter : Towards live vaccines and whole-cell biocatalysis

   Författare :Martin Gustavsson; Gen Larsson; Per Berglund; Andres Veide; Jan-Willem de Gier; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; AIDA-autotransporter; Escherichia coli; live vaccines; surface expression;

   Sammanfattning : The area of surface expression has gathered a lot of interest from research groups all over the world and much work is performed in the area. Autotransporters have been used for surface expression in Gram-negative bacteria. LÄS MER

4. 3. Bioreduction of Carbonyl Compounds to Chiral Alcohols by Whole Yeast Cells: Process Optimisation, Strain Design and Non-Conventional Yeast Screening

   Författare :Michael Katz; Teknisk mikrobiologi; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; bicyclic diketone; carbonyl; yeast; candida; cerevisiae; reductase; ketone; reduction; whole cell; YMR226c; YDR368w; Microbiology; bacteriology; virology; mycology; Biokemisk teknik; Biochemical technology; mykologi; virologi; Mikrobiologi; bakteriologi;

   Sammanfattning : Chiral building blocks are needed for the production of drugs and fine chemicals, which requires the use of several synthetic routes to produce a specific enantiomer of interest. One promising approach to introduce chirality into molecules is the stereo-selective whole cell bioreduction of carbonyl compounds or ketones to the corresponding chiral alcohols. LÄS MER

5. 4. Biocatalytic transamination with recombinant Saccharomyces cerevisiae: Challenges and opportunities

   Författare :Nora Weber; Teknisk mikrobiologi; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Saccharomyces cerevisiae; transamination; whole-cell biocatalysis; pyruvate; PLP; thiamine; reduction;

   Sammanfattning : Chiral building blocks are important molecules for synthesis of fine chemicals and pharmaceuticals. Biocatalysis has gained in relevance over traditional organic methods for synthesis of chiral compounds, as reactions can be performed at high enantio-selectivity and purity with environmentally friendly, simple, and cheap methods. LÄS MER

7. 5. Biosynthesis of capsaicinoids by recombinant Saccharomyces cerevisiae

   Författare :Nina Muratovska; Teknisk mikrobiologi; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; capsaicinoids; nonivamide; baker’s yeast; Capsicum; Saccharomyces cerevisiae; Pseudomonas putida; lignin-derived substrates; vanillin aminotransaminase; reductive amination; vanillylamine; N-Acyltransferase; CoA-ligase; amide; whole-cell bioconversion; TRPV1 receptor; ion channel; calcium dye Fluo-4; flow cytometry;

   Sammanfattning : Throughout history, people have used products from different microorganisms and plants for many medicinal and nutritional applications. Chilli peppers have been used for spicing up food, but also for their pharmacological properties. LÄS MER