Sökning: "whole genome amplification"

Visar resultat 26 - 30 av 30 avhandlingar innehållade orden whole genome amplification.

  1. 26. Developing methods for mapping genetic heterogeneity in tumors : from bulk to single-cell resolution

    Författare :Michele Simonetti; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Tumors are complex ecosystems composed of billions of cells that constantly evolve over time. Intra-tumor heterogeneity (ITH) represents the diversity of this complex environment quantifiable both at the genetic and phenotypic level. Recently, next-generation sequencing (NGS) costs have dropped and NGS has become the standard tool for studying ITH. LÄS MER

  3. 27. Predictive Markers of Treatment Resistance in Head and Neck Squamous Cell Carcinoma

    Författare :Fredrik Jerhammar; Karin Roberg; Roland Grafström; Agneta Jansson; Keith D. Hunter; Linköpings universitet; []
    Nyckelord :;

    Sammanfattning : Head and neck cancer is a common malignancy with approximately 600 000 new cases yearly. Disappointingly, the overall survival has not increased over the last decades. The concept of personalized medicine, i.e. LÄS MER

  4. 28. Investigating novel mechanisms for transcriptional memory using genetic engineering

    Författare :Yerma Pareja Sanchez; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Transcriptional memory is phenomenon that has gained interest due to its potential impact on human health. With our work, we wanted to contribute to the understanding of its regulation. To do so, we performed genome-wide knockout screens in both budding yeast and human cells. LÄS MER

  5. 29. Delection mapping of human 3p in major epithelial types of cancer and fine localization of candidate tumor suppressor genes

    Författare :Jian Liu; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Tumor suppressor gene; epithelial carcinoma; human chromosome 3p;

    Sammanfattning : Allele loss and deletion mapping using microsatellite markers and the detection of homozygous deletions represented until now the most powerful method to localize potential TSGs. Loss of heterozygosity (LOH) involving several chromosome 3p regions accompanied by chromosome 3p deletions are detected in almost 100% of renal cell carcinoma (RCC), small (SCLCs) and more than 90% of non-small (NSCLC) cell lung cancers. LÄS MER

  7. 30. Extremely drug resistant E. coli and K. pneumoniae : novel approaches against their spread and related infections

    Författare :Angela Camporeale; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Antibiotic resistance in Enterobacteriaceae is a well-established hindrance and the spread of pathogenic high-risk clones harboring extended-spectrum -lactamases (ESBLs) and carbapenemases is posing a global health challenge, due to very less treatment alternatives. Particular lineages from the major representative species E. coli and K. LÄS MER