Sökning: "viral kinetics"

Visar resultat 1 - 5 av 56 avhandlingar innehållade orden viral kinetics.

1. 1. Interaction Characteristics of Viral Protease Targets and Inhibitors : Perspectives for drug discovery and development of model systems

   Författare :Cynthia F Shuman; Jordan J N Tang; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; viral proteases; biomolecular interactions; kinetics; thermodynamics; biosensors; drug discovery; Biokemi; Biochemistry; Biokemi; Biokemi; Biochemistry;

   Sammanfattning : Viral proteases are important targets for anti-viral drugs. Discovery of protease inhibitors as anti-viral drugs is aided by an understanding of the interactions between viral protease and inhibitors. This thesis addresses the characterization of protease-inhibitor interactions for application to drug discovery and model system development. LÄS MER

3. 2. Nucleotide Binding to Trimeric dUTPase: Studies on Recombinant Enzymes using Kinetic and Spectroscopic Methods

   Författare :Johan Nord; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; C-terminus; dUTPase; EIAV; Escherichia coli; flexibility; Homo sapiens; kinetics; NMR; nucleotide metabolism; rate constants of lig-and binding; production of recombinant enzyme; dUTP; specificity; Biochemistry; Metabolism; Biokemi;

   Sammanfattning : Recombinant dUTPase from the bacterium Escherichia coli, the retrovirus equine infectious anemia virus (EIAV) and Homo sapiens have been made available for physico-chemical characterisation. The protocols developed have also proven to be suitable for production of enzymes modified by isotope labelling or other methods. LÄS MER

4. 3. Novel Procedures for Identification and Characterization of Viral Proteases Inhibitors

   Författare :Angelica Ehrenberg; Helena Danielson; Celia Schiffer; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; drug discovery; viral proteases; inhibitors; Hepatitis C; NS3; cytomegalovirus; Biokemi; Biochemistry;

   Sammanfattning : Viral proteases are often considered to be attractive drug targets because of their crucial function in the viral replication machinery. In order to increase our knowledge of these important targets and to contribute to the discovery and development of new antiviral drugs, the proteases from hepatitis C virus (HCV) and human cytomegalovirus (HCMV) have been produced and their interactions with inhibitors and fragments have been characterized, using enzyme inhibition and SPR biosensor based interaction assay. LÄS MER

5. 4. Homotrimeric dUTPases : Principles of Catalysis and Inhibitor Design

   Författare :Lorena Gonzalez Palmén; Jan Kvassman; Dolores Gonzalez-Pacanowska; Högskolan i Kalmar; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; dUTPases; kinetics; ground state destabilization; inhibitors; Biochemistry; Biokemi; Natural Science; Naturvetenskap;

   Sammanfattning : The ubiquitous enzyme dUTPase hydrolyzes dUTP into dUMP and pyrophosphate, preventing DNA fragmentation and cell death due to accumulation of dUTP. Inhibitors of dUTPase could serve as drugs in the treatment of cancers and infectious diseases. This thesis presents five studies. LÄS MER

7. 5. Viral infections in children with cancer

   Författare :Anna Lindblom; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Viral infections are the most common cause of infection morbidity in children. Nevertheless, few studies have been devoted to exploring the viral panorama in children receiving chemotherapy for cancer. LÄS MER