Sökning: "universal stress protein"

Hittade 5 avhandlingar innehållade orden universal stress protein.

  1. 1. Engineering yeast for improved recombinant protein production

    Författare :Yanyan Wang; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; point mutation; genome-scale modeling; recombinant protein; omics analysis; CRISPR Cas9; protein secretion; Saccharomyces cerevisiae;

    Sammanfattning : Recombinant proteins are broadly used from basic research to therapeutic development and include industrial enzymes and pharmaceutical proteins. The increasing demand for improved production and enhanced quality of recombinant proteins requires robust biotech-based strategies to overcome the limitations of protein extraction from natural sources. LÄS MER

  2. 2. General stress proteins: Novel function and signals for induction of stationary phase genes in E.coli

    Författare :Örjan Persson; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; general stress response; universal stress protein; sigma S; DNA damage; fructose-6-phosphate; ruvC;

    Sammanfattning : Survival during conditions when nutrients become scarce requires adaptation and expression of genes for maintenance in order for the cell to survive. Among the numerous proteins involved in adaptation and regulation under these conditions, the stationary phase sigma factor, σS, and the Universal stress proteins contribute to survival and bestow the cell with general stress protective functions during growth arrest. LÄS MER

  3. 3. The use of protein biomarkers in ecotoxicology : Studies of oxidative and genotoxic stress in the blue mussel (Mytilus edulis)

    Författare :Andreas Prevodnik; Michael Tedengren; Tomas Bollner; Kari Lehtonen; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biology; Biologi; Ecotoxicology; ekotoxikologi;

    Sammanfattning : Many environmental pollutants, heavy metals and polyaromatic hydrocarbons (PAHs) among them, are toxic by oxidative stress. Oxidative stress may be defined as a state in an organism when its inherent capacity to handle oxyradicals is surpassed, and it may result in peroxidation of lipids, and damage to proteins and DNA. LÄS MER

  4. 4. Impact of glucose feed rate on productivity and recombinant protein quality in Escherichia coli

    Författare :Anna Maria Sandén; Gen Larsson; Gunnar Lidén; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Escherichia coli; recombinant protein production; fed-batch; feed profile; specific growth rate; Biokemi; Biochemistry; Biokemi;

    Sammanfattning : The goal of this work was to contribute to the fed-batch process optimisation task by deriving parameters that have considerable impact on productivity as well as product quality The chosen parameters were I) the design of the glucose feed profile, II) the choice of induction strategy, with respect to the method of addition, and III) the time of the induction, with respect to the specific glucose consumption rate.The present fed-batch experiments using the lacUV5-promoter, for production of b-galactosidase, have shown that a high glucose feed rate gives a specific production rate, qp, that is twice as high, after induction, compared to a feed rate that is 2. LÄS MER

  5. 5. Cell-to-cell communication and virulence in Vibrio anguillarum

    Författare :Kristoffer Lindell; Debra Milton; Karl Klose; Umeå universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; vibrio anguillarum; quorum sensing; stress response; keratocyte; molekylärbiologi; Molecular Biology;

    Sammanfattning : Quorum sensing (QS) is a type of cell-to-cell communication that allows the bacteria to communicate via small molecules to coordinate activities such as growth, biofilm formation, virulence, and stress response as a population. QS depends on the accumulation of signal molecules as the bacterial population increases. LÄS MER