Sökning: "tyrosyl radical"

Visar resultat 1 - 5 av 17 avhandlingar innehållade orden tyrosyl radical.

  1. 1. Essential conserved active site residues in class Ia ribonucleotide reductase from Escherichia coli : identification of radical reaction intermediates and radical transfer intermediates

    Författare :Annika Persson; Marc Fontecave; Stockholms universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Molecular Biology; molekylärbiologi;

    Sammanfattning : The enzyme ribonucleotide reductase catalyzes the reduction of ribonucleotides to the corresponding deoxyribonucleotides, the building blocks of DNA. The class Ia enzyme from Escherichia coli consists of two homodimeric subunits denoted protein R1 and protein R2, each inactive alone. LÄS MER

  2. 2. Activation and inhibition of diiron and iron-manganese ribonucleotide reductases

    Författare :Ana Popović-Bijelić; Astrid Gräslund; Anders Hofer; Stockholms universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; class Ic; C. trachomatis; cytotoxicity; KP772; lanthanum; manganese–iron cofactor; metal complex; oligopeptide inhibitor; ribonucleotide reductase; Triapine; tyrosyl radical; Biophysics; biofysik;

    Sammanfattning : Ribonucleotide reductase (RNR) catalyses the reduction of ribonucleotides to deoxyribonucleotides. In conventional class I RNRs the active site is located in the R1 subunit, and the R2 subunit contains a diiron cofactor and a stable tyrosyl radical essential for activity. LÄS MER

  3. 3. Radical generation and stabilisation in ribonucleotide reductase R2

    Författare :Martin Högbom; Lawrence Que; Stockholms universitet; []
    Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biochemistry; Biokemi; biokemi; Biochemistry;

    Sammanfattning : Diiron carboxylate proteins contain a cofactor that consists of two iron atoms coordinated by carboxylate and histidine ligands. These proteins perform a multitude of chemical reactions in the cell that generally involve activation of molecular oxygen at the diiron site. LÄS MER

  4. 4. Electron paramagnetic resonance and biochemical studies of red-ox properties of the diferric/radical center in mouse and Mycobacterium tuberculosis ribonucleotide reductase R2 proteins

    Författare :Albert Davydov; Marc Fontecave; Stockholms universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Biophysics; biofysik;

    Sammanfattning : Ribonucleotide reductase (RNR) catalyses the reduction of all four ribonucleotides to the corresponding deoxyribonucleotides that are used by the cells for DNA synthesis. The active enzyme from mouse, Mycobacterium tuberculosis (M. tuberculosis) and aerobically grown E. LÄS MER

  5. 5. Biophysical studies of ribonucleotide reductase

    Författare :Margareta Sahlin; Stockholms universitet; []
    Nyckelord :;

    Sammanfattning : Ribonucleotide reductase (EC 1.17.4) catalyses the enzymatic reduction of ribonucleotides to their corresponding deoxyribo- nucleotides, the monomeric precursors for the DNA synthesis in all living cells. All ribonucleotide reductases so far Isolated from different species have been found to contain metal ions, either iron, cobolt or manganese. LÄS MER