Sökning: "two-dimensional gel electrophoresis"

Visar resultat 16 - 20 av 65 avhandlingar innehållade orden two-dimensional gel electrophoresis.

1. 16. Glucocorticoid receptor function : interactions, mutants and ligand responses

   Författare :Erik Hedman; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :Glucocorticoid receptor; glucocorticoids; cross-talk; GR interacting proteins; Flt3; mass spectrometry; 2D gel electrophoresis;

   Sammanfattning : The protein that is investigated in this thesis is the glucocorticoid receptor (GR), which belongs to the nuclear hormone receptor superfamily of ligand activated transcription factors. The nuclear receptors share three conserved structural domains, the N-terminal transactivating domain, the central DNA-binding domain and the C-terminal ligand-binding domain. LÄS MER

3. 17. Bronchoalveolar lavage and serum protein patterns in healthy individuals and sarcoidosis patients : a proteomics approach

   Författare :Fariba Sabounchi Schütt; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :Sarcoidosis; BALF; serum; proteomics; 2-D electrophoresis; protein-spot pattern; protein identification;

   Sammanfattning : The etiology of sarcoidosis remains unknown. Although biochemical changes are frequently observed in sarcoidosis, the problem of finding distinct biomarkers remains. Only a few markers are defined in this disease. LÄS MER

4. 18. Mass spectrometry in peptide and tumor marker analysis

   Författare :Ann-Charlotte Bergman; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Integration of preparative protein and peptide separations with analytical techniques, in particular mass spectrometry (MS), is important. This thesis describes preparation via capillary electrophoresis and two-dimensional (2-D) gel electrophoresis for identification and structure determination of polypeptides. LÄS MER

5. 19. Proteome studies, with emphasis on the kidney glomerulus

   Författare :Sam Tryggvason; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Proteomics aims to link genes to protein expression and function. Proteomics is a very versatile and technology-dependent discipline and the development of mass spectrometry-based proteomics has advanced the understanding of the complex and dynamic nature of proteomes. LÄS MER

7. 20. Protein trafficking in the cell envelope of Escherichia coli : Identification and characterisation of a novel chaperone

   Författare :Hansjörg Götzke; Daniel Daley; Hans-Georg Koch; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; biokemi;

   Sammanfattning : The cell envelope of Gram-negative bacteria, like Escherichia coli, is composed of a cytoplasmic membrane, a periplasmic space containing a peptidoglycan layer and an outer membrane. About 30 % of all proteins are localised in the cell envelope. LÄS MER