Sökning: "trypsin"
Visar resultat 16 - 20 av 91 avhandlingar innehållade ordet trypsin.
16. C-reactive protein in clinical practice with special regard to infectious diseases
Sammanfattning : A new simple affinity purification method for human CRP using Arginine-Sepharose 4B was developed with a recovery of >90 % and containing < 5-10 % contaminating proteins. In a multicenter study including 607 consecutive patients in general practice, erythrocyte sedimentation rate (ESR) and serum CRP were compared. LÄS MER
17. Aspects of proteinase-activated receptor-2: A sensor of enzymatic activity at the cell surface
Sammanfattning : Information about extracellular proteolytic activity is transduced to the cell by a transmembrane G-protein coupled receptor subfamily. These receptors are activated by proteolytic cleavage in the extracellular amino-terminal domain and are thus termed proteinase-activated receptors (PARs). LÄS MER
18. Studies of a Proteinase-activated Cell Surface Receptor
Sammanfattning : The G protein-coupled receptors form one of the largest families of proteins involved in signalling between cells. It contains receptors for molecules as diverse as biogenic amines, glycoproteins and enzymes. The manuscripts included in this thesis together describe the initial characterisation of a previously unknown receptor of this class. LÄS MER
19. Miniaturized Techniques for Protein Analysis
Sammanfattning : Proteins are a highly diversified group of molecules, andfor their study, advanced analytical tools are required. Inparticular, a need for high-throughput techniques has emergedin order to enable the characterization of large sets ofproteins. LÄS MER
20. Membrane protein proteomics - Novel method for membrane protein identification and quantification
Sammanfattning : Membrane proteins are fairly refractory to digestion especially by trypsin. Less specific proteases like elastase and pepsin are much more effective. However database searching using non-tryptic peptides is much less effective due to the lack of charge localisation at the N- and C-termini and the absence of sequence specificity. LÄS MER