Sökning: "total internal reflection microscopy"

Visar resultat 1 - 5 av 30 avhandlingar innehållade orden total internal reflection microscopy.

1. 1. Colloidal interactions obtained from total internal reflection microscopy measurements and scattering data

   Författare :Moheb Nayeri; Göteborgs universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; total internal reflection microscopy; colloidal stability; van der Waals interaction; Debye length; surfactants; depletion; scattering; colloidal stability;

   Sammanfattning : The scattering of radiation can be used to extract information about the interactions between colloidal (10^{-7}-10^{-3} cm radius) particles suspended in liquids. As colloidal interactions incorporate entropic effects they are weak and while system specific they are governed by a number of general mechanisms. LÄS MER

3. 2. DNA-Controlled Lipid-Membrane Fusion

   Författare :Lisa Simonsson; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; exocytosis; vesicles; DNA; FRET; fluorescence resonance energy transfer; model system; total internal reflection fluorescence microscopy; TIRF microscopy; self-assembly; site-specificity; diffusion; biomembranes; lipid bilayer; membrane fusion;

   Sammanfattning : Membrane fusion is essential for nerve-cell communication, for protein transport between cell organelles and the cell-membrane and for enabling the merger between virus and host membranes during virus infection. We have demonstrated that short DNA oligonucleotides, membrane-attached via CH in an orientation that mimics the overall zipperlike architecture of fusion-inducing proteins, induce fusion of both suspended vesicles and vesicles site-specifically tethered to SLBs. LÄS MER

4. 3. Lipid Vesicle Fusion: Investigation, Generation and Manipulation of Cell-Membrane Mimics

   Författare :Lisa Simonsson; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; diffusion; spreading lipid bilayers; cell mimic; micromanipulation; lipid bilayer; microfluidics; site-specificity; DNA; membrane-receptor interactions; total internal reflection fluorescence microscopy; model system; self-assembly; TIRF microscopy; FRET; exocytosis; vesicles; fluorescence resonance energy transfer; biomembranes; amperometry; membrane fusion;

   Sammanfattning : Membrane fusion is essential for nerve-cell communication, for protein transport between cell organelles and the cell-membrane and for enabling the merger between virus and host membranes during virus infection. In this work, cell-membrane mimics were constructed and evaluated as models for studies of the membrane-fusion process. LÄS MER

5. 4. Fluorescence microscopy studies of interactions between monomeric α-Synuclein and lipid membranes

   Författare :Alexandra Andersson; NanoLund: Centre for Nanoscience; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Total internal reflection fluorescence microscopy; single vesicle imaging; single molecule imaging; protein-lipid interactions; α-Synuclein;

   Sammanfattning : α-Synuclein (α-Syn), is an intrinsically disordered protein present in the substantia nigra part of the brain. α-Syn is commonly known to associate with lipid membranes containing anionic lipids. LÄS MER

7. 5. Unraveling the molecular mechanisms of herpes simplex virus attachment and release using cell membrane mimics

   Författare :Nadia Peerboom; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; total internal reflection fluorescence microscopy; glycosaminoglycans; atomic force microscopy; binding kinetics; single particle tracking; herpes simplex virus;

   Sammanfattning : The herpes simplex virus is a widespread human pathogen, most commonly known for causing cold sores. Its infection cycle is initiated with the formation of multiple bonds between viral glycoproteins and cellular glycosaminoglycans, which are long polysaccharide chains found close to the cell surface. LÄS MER