Sökning: "tof-sims"

Visar resultat 11 - 15 av 48 avhandlingar innehållade ordet tof-sims.

  1. 11. Development and Evaluation of Preparation Methods for the Analysis of Cell Membranes Using Time-of-Flight Secondary Ion Mass Spectrometry

    Författare :Jakob Malm; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; salt; cryofixation; TOF-SIMS; supported phospholipid bilayer; freeze drying; vacuum; cells; POPC; glutaraldehyde;

    Sammanfattning : The aim of this work was to evaluate and develop methods for preparing dry samples for TOF-SIMS analysis of cell membranes. For correct interpretation of the analysis data, an understanding of the effects that the preparation method has on the morphology and chemical structure of the sample must first be obtained. LÄS MER

  3. 12. Detection of Lipids and Proteins on Biological Surfaces using Imaging Mass Spectrometry

    Författare :Louise M Carlred; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; ToF-SIMS; GM1; cholesterol; Amyloid-β; phospholipids; Imaging Mass Spectrometry; Alzheimer’s disease; sulfatides; QCM-D; MALDI;

    Sammanfattning : Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is a technique that can be used for imaging the spatial distribution of many different molecules at the same time. It is very sensitive for detection of small biomolecules, such as lipids, whereas larger biomolecules, such as peptides and proteins, cannot be detected as intact entities due to fragmentation. LÄS MER

  4. 13. Towards Anti-Virulence Antimicrobials, Discovery and Development of Sortase A Inhibitors and Investigations of Bacterial Phenotypes

    Författare :Patrick M. Wehrli; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Sortase A; SrtA; Inhibitors; Anti-virulence; Bacterial analysis; FTIR spectroscopy; Bacterial phenotyping; Design of Experiment; Multivariate Data Analysis; PCA; ToF-SIMS; Time-of-flight secondary-ion-mass-spectrometry; Lipid analysis; ppGpp; Stringent response;

    Sammanfattning : Antibiotic resistance is an emerging and serious threat to public health. Immediate actions are required to preserve current antibiotics while intensifying research efforts towards the development of new effective therapeutics. LÄS MER

  5. 14. Analysis of phospholipids in cellular membranes with LC and imaging mass spectrometry

    Författare :Ingela Lanekoff; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Mass spectrometry; TOF-SIMS; Phospholipid; Cells; Anammox;

    Sammanfattning : Imaging mass spectrometry enables the creation of molecule specific images from the surface of a solid sample in vacuum. To solve the issue of bringing single cells into vacuum without altering their native distribution of molecules, a freeze fracture device that fits the time of flight secondary ion mass spectrometry (TOF-SIMS) IV instrument has been developed. LÄS MER

  7. 15. PROBING SECRETORY VESICLES AND LIPOSOME MODEL SYSTEMS USING NANOSCALE ELECTROCHEMISTRY AND MASS SPECTROMETRY

    Författare :Jelena Lovric; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; neuroendocrine cells; NanoSIMS; liposomes; neurotransmitters; large dense core vesicles; Exocytosis; ToF-SIMS; vesicle electrochemical cytometry; electrochemistry; amperometry;

    Sammanfattning : Cellular communication is based on the process of exocytosis, regulated release of chemical messengers into the extracellular space. These messengers, neurotransmitters, are packed into vesicles that during exocytosis fuse with the plasma membrane and release their content. LÄS MER