Sökning: "thermostability"

Visar resultat 1 - 5 av 28 avhandlingar innehållade ordet thermostability.

  1. 1. Characterization and engineering of carbohydrate-active enzymes for biotechnological applications

    Författare :Noor Hassan; Christina Divne; Eva Nordberg Karlsson; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; se conversion; galacto-oligosaccharides; thermostability; propeptide; Bioteknologi; Biotechnology;

    Sammanfattning : Extremozymes are enzymes produced by microorganisms that live in extreme habitats. Due to their higher stability, extremozymes is attracting interest as biocatalysts in various industrial processes. In this context, carbohydrate-active extremozymes can be used in various processes relevant to the paper, food and feed industry. LÄS MER

  2. 2. From Time-Averaged to Time-Resolved Crystallography: Studies on Superoxide Dismutase and Myoglobin

    Författare :Thomas Ursby; Lunds universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; superoxide dismutase; Bayesian statistics; electron difference maps; synchrotron; docking site; photolysis; myoglobin; Time-resolved crystallography; Laue; thermostability; Sulfolobus solfataricus; Molecular biophysics; Molekylär biofysik;

    Sammanfattning : We have studied protein motions with nanosecond time-resolved crystallography. The release of carbon monoxide from its complex with myoglobin (MbCO) was triggered by nanosecond laser pulses. X-ray data were collected with time delays ranging from 4 ns to 1.9 ms using the Laue method, at the European Synchrotron Radiation Facility in Grenoble. LÄS MER

  3. 3. Advancements in Firefly Luciferase-Based Assays and Pyrosequencing Technology

    Författare :Jonas Eriksson; KTH; []
    Nyckelord :bioluminescence; osmolytes; glycine betaine; thermostability; firefly luciferase; inorganic pyrophosphatase; inorganic pyrophosphate; Pyrosequencing technology; secondary DNA-structures; Sequenase; Klenow-polymerase; reaction rates; temperature;

    Sammanfattning : Pyrosequencing is a new DNA sequencing method relying on thesequencing-by-synthesis principle and bioluminometric detectionof nucleotide incorporation events. The objective of thisthesis was improvement of the Pyrosequencing method byincreasing the thermal stability of firefly luciferase, and byintroducing an alternative DNA polymerase and a new nucleotideanalog. LÄS MER

  4. 4. The conserved methionines in the chloroplast small heat shock protein - Role in chaperone-substrate interactions and effects of methionine sulfoxidation on chaperone activity

    Författare :Niklas Gustavsson; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Metabolism; Biochemistry; oxidative stress; thermostability; temperature stress; stress tolerance; sHsp; protein aggregation; molecular chaperone; Heat stress; methionine sulfoxidation; Biokemi; metabolism; Plant biochemistry; Växtbiokemi;

    Sammanfattning : The chloroplast-localized small heat shock protein (sHsp) Hsp21 belongs to the family of alpha-crystallin like sHsps which form large oligomeric structures and protect partly unfolded aggregation-prone proteins against aggregation. The main focus for this thesis has been on the highly conserved methionines in Hsp21. LÄS MER

  5. 5. Exploring thermostable glycoside hydrolases: Amylases and beta-glucosidases

    Författare :Pernilla Turner; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Bioteknik; Biotechnology; Proteiner; beta-glucosidase; thermostability; activity; cyclodextrinase; enzymology; crystallization; enzymologi; expression; glycoside hydrolase; Proteins; transglycosylation;

    Sammanfattning : Two putative cyclodextrinases (CDases) were amplified from two moderate thermophiles isolated in Iceland: Anoxybacillus flavithermus and Laceyella sacchari. They were cloned, expressed and characterized. The expression of the former was optimized in E. LÄS MER