Sökning: "temporal bone preparation"

Hittade 4 avhandlingar innehållade orden temporal bone preparation.

1. 1. Hair cell and organ of corti responses to normal and intense acoustic stimulation

   Författare :Anders Fridberger; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :Cochlea; outer hair cells; calcium; pressure changes; micromechanics; noise-induced hearing loss; fluorescent indicators; laser interferometry; temporal bone preparation; guinea pig;

   Sammanfattning : The principal aims of the studies described in this thesis were to develop an in vitro model for studying acoustic overstimulation at the cellular level, to define the electrical and mechanical response characteristics of the perfused temporal bone preparation, and to investigate the effects of intense sound stimulation on the calcium levels of the hair cells in the intact hearing organ. In the in vitro model for acoustic overstimulation, isolated cochlear outer hair cells were subjected to a pressure jet emanating from a glass micropipette aimed at the cell body. LÄS MER

3. 2. Imaging the organ of Corti in vitro and in vivo

   Författare :Igor Tomo; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Recent developments in imaging techniques, namely confocal laser scanning microscopy, offer new tools in exploration of the structure and function of the inner ear. The optical sectioning property of the confocal microscope enables investigation of living cells deeper within a labeled intact tissue. LÄS MER

4. 3. Physiological aspects of cochlear excitation and neurotransmitter release

   Författare :Wanje Jäger; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :Cochlea; cochlear microphonics; summating potential; vibratory response; tetracaine; aspartate; glutarnate; neurotransmitter; noise; hearing loss;

   Sammanfattning : Using an in vitro model of the guinea pig, the temporal bone preparation, cochlear physiology was studied. By perfusion of the scala tympani with oxygenated culture medium, the responses of the hearing organ to sound stimuli were maintained over several hours and the functional state was followed by monitoring the electrophysiological parameters cochlear microphonics (CM) and summating potential (SP). LÄS MER

5. 4. Image processing methods to study the living hearing organ

   Författare :Miriam von Tiedemann; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : In this thesis we are developing image processing methods allowing one to probe the structure and function of the mammalian hearing organ, an intricate cellular assembly with a highly specialized three-dimensional organization. Confocal as well as electron microscopy was used for image acquisition. LÄS MER