Sökning: "surface-based biosensing"

Visar resultat 1 - 5 av 8 avhandlingar innehållade orden surface-based biosensing.

1. 1. Physical characterization of exosomes and other extracellular vesicles using surface-based sensing

   Författare :Deborah Rupert; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; nanoparticle tracking analysis; exosomes; scattering; surface-based biosensing; optical density; cluster of differentiation 63 CD63 ; liposomes; label-free; fluorescence; surface plasmon resonance; Extracellular vesicles; specificity; antibody; lipid content; single particle; quantification; size distribution; optical waveguide microscopy;

   Sammanfattning : Extracellular vesicles (EVs) are hundred of nanometer sized particles secreted by almost all cell types. Exosomes are one type of EVs today intensively investigated due to the increased evidence suggesting that they play important roles as communicating vehicles in a broad range of physiological processes, pathological conditions and disease diagnostics. LÄS MER

3. 2. Tethered Lipid Vesicles as Nanoscale Sensor Elements

   Författare :Seyed Tabaei; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; aquaglyceroporin; lipid vesicles; total internal reflection fluorescence TIRF microscopy; cell membrane; aquaporin; pore formation; surface plasmon resonance SPR ; phospholipase A2; antiviral peptide; lipid bilayer; biosensing; microfluidics;

   Sammanfattning : The primary aim of this thesis work has been to explore and advance the potential of surface tethered lipid vesicles as a platform for studying in particular the following three biomembrane-related processes: trans-membrane transport, membrane-peptide interactions and the activity of membrane active enzymes. First, a method based on surface plasmon resonance (SPR) spectroscopy was developed for studying passive diffusion processes as well as the membrane-protein mediated transport of small non electrolyte molecules across lipid bilayers. LÄS MER

4. 3. Nanoplasmonic Biosensing - Exploring Unique Possibilities

   Författare :Magnus Jonsson; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; biosensor; nanoholes; nanopores; nanofabrication; localized surface plasmon resonance; quartz crystal microbalance; nanoplasmonics; structural sensing; artificial cell membrane;

   Sammanfattning : Bioanalytical sensors are indispensible tools in medical diagnostics and drug discovery as well as for life science research, environmental monitoring and food safety. In essence, they are used to detect and determine the concentrations of specific biomolecules in complex mixtures. LÄS MER

5. 4. Studies of Glycosaminoglycan Interactions - Surface Immobilization Strategies and Biosensing Applications

   Författare :Noomi Altgärde; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; glycosaminoglycans; interaction studies; growth factor; QCM-D; virus glycoprotein; SPR; surface immobilization;

   Sammanfattning : Many important biological functions of glycosaminoglycans (GAGs) have been highlighted in research literature during recent years. GAGs often serve as function-bearing structural elements in the extracellular matrix (ECM), but are also constituents of the cell membrane. GAGs take part in various biological mechanisms, e.g. LÄS MER

7. 5. Concatemerization of Synthetic Oligonucleotides

   Författare :Lu Sun; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; gel electrophoresis; DNA-binding; QCM-D; DNA concatemer; hybridization; DNA formation; self-assembly;

   Sammanfattning : DNA nanotechnology has become an important research field because of its advantages in high predictability and accuracy of base-paring recognition. For example, the DNA concatemer, one of the simplest DNA constructs in shape, has been used to enhance signals in biosensing. LÄS MER