Sökning: "superfusion"

Visar resultat 1 - 5 av 8 avhandlingar innehållade ordet superfusion.

1. 1. Studies of individual pancreatic -cells : Electrophysiological analysis of rhythmic behaviour and development of new techniques

   Författare :Staffan Dryselius; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Cell biology; Diabetes; pancreatic beta-cells; insulin release; signal transduction; cytoplasmic calcium; oscillations; patch clamp; ATP-regulated potassium channels; voltage-dependent calcium channels; metabolism; calcium ion; strontium ion; manganese ion; wavelets; de-noising; pattern recognition; superfusion; solution changes; microscopic labelling; Cellbiologi; Cell biology; Cellbiologi; medicinsk cellbiologi; Medical Cell Biology;

   Sammanfattning : The insulin concentration in blood varies periodically, which is believed to prevent down-regulation of the hormone receptors. Loss of the regular insulin oscillations is considered to be an early sign of developing diabetes. LÄS MER

3. 2. The Multifunctional Pipette. A Microfluidic Technology for the Biosciences

   Författare :Alar Ainla; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; microfluidic superfusion; microfluidic temperature sensing.; microfluidic dilution; hydrodynamic flow confinement; microfluidic mixer; supported lipid membranes; single-cell analysis; Microfluidics; microfabrication; PDMS;

   Sammanfattning : The theme of the work described in this thesis is the generation and application of liquid microenvironments in chemistry and bioscience using microfluidic devices. First, a computer controlled multi-stage dilution system to generate time-dependent chemical waves was developed, and its application was demonstrated on model biomembranes. LÄS MER

4. 3. On methods for probing structure and function of membrane proteins

   Författare :Erik Jansson; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; proteomics; patch-clamp; mass spectrometry.; proteoliposomes; microfluidic superfusion; cholesterol; TRPV1; limited proteolysis; Membrane proteins; fluorescence;

   Sammanfattning : The determination of structure–function relationships in membrane proteins plays a key role for the understanding of molecular mechanisms in biological systems, and may provide information on how these systems can be modulated by pharmaceuticals. The aim of the work described herein was to develop methods for studying structure and function of membrane proteins, ideally in combination. LÄS MER

5. 4. Microfluidics based techniques for electrophysiological studies of cells

   Författare :Aikeremu Ahemaiti; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

   Sammanfattning : This thesis work investigates the application of microfluidics to perform electrophysiological studies on cells, including investigations of the effect of cholesterol on the dynamic ion permeability of TRPV1 ion channels, and the application of a microfluidic device, the multifunctional pipette, in electrophysiological studies on brain slices. In the first part of this thesis, Chinese hamster ovary (CHO) cells overexpressing the TRPV1 ion channel were used in a dynamic ion permeability study, where the activation properties of the TRPV1 ion channel were investigated using the patch clamp technique after depletion of membrane cholesterol. LÄS MER

7. 5. Patch-clamp studies of the GABAA receptor using microfluidic methods

   Författare :Maria Millingen; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; ion channel; microfluidics; GABAA receptor;

   Sammanfattning : Ion channels are membrane proteins that passively transport ions. When activated, conformational changes within the protein lead to opening of a channel pore, and the flow of ions causes a change in the membrane potential. This is the fundamental process behind the generation and transduction of nerve impulses. LÄS MER