Sökning: "subcellular localization"

Visar resultat 1 - 5 av 110 avhandlingar innehållade orden subcellular localization.

  1. 1. Antibody-based subcellular localization of the human proteome

    Författare :Marie Skogs; Emma Lundberg; Hans Blom; Cecilia Williams; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Human proteome; Subcellular localization; Organelles; Immunofluorescence; Fixation; Permeabilization; Antibody validation; Bioteknologi; Biotechnology;

    Sammanfattning : This thesis describes the use of antibodies and immunofluorescence for subcellular localization of proteins. The key objective is the creation of an open-source atlas with information on the subcellular location of every human protein. LÄS MER

  3. 2. Towards subcellular localization of the human proteome using bioimaging

    Författare :Charlotte Stadler; Emma Lundberg; Carolina Wählby; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Antibody; antibody validation; automated image analysis; automated microscopy; cell line; confocal microscopy; fixation; green fluorescent protein GFP ; immunofluorescence IF ; organelle; protein expression; siRNA; subcellular localization;

    Sammanfattning : Since the publication of the complete sequence of the human genome in 2003 there has been great interest in exploring the functions of the proteins encoded by the genes. To reveal the function of each and every protein, investigation of protein localization at the subcellular level has become a central focus in this research area, since the localization and function of a protein is closely related. LÄS MER

  4. 3. Leukotriene C4 synthase : studies on oligomerization and subcellular localization

    Författare :Jesper Svartz; Mats Söderström; Sven Hammarström; Colin Funk; Linköpings universitet; []
    Nyckelord :MEDICINE; MEDICIN;

    Sammanfattning : Leukotrienes (LTs) are polyunsaturated fatty acid derivatives formed by oxygenation of arachidonic acid via the 5-lipoxygenase (5-LO) pathway. Upon activation of inflammatory cells 5-LO is translocated to the nuclear envelope where it converts arachidonic acid to the unstable epoxide LTA4. LÄS MER

  5. 4. Large-scale prediction of protein subcellular localization : sequence-based methods and applications

    Författare :Olof Emanuelsson; Alfonso Valencia; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Biophysics; Biofysik; Biochemistry; Biokemi; Biophysics; biofysik;

    Sammanfattning : .... LÄS MER

  7. 5. Subcellular localization and signalling of Brutons tyrosine kinase (Btk)

    Författare :Leonardo Vargas-Vallejo; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :B-cells; Brutons tyrosine kinase; X-linked agammaglobulinemia; pleckstrin homology domain; cytoskeleton; nucleocytoplasmic shuttling; caveolin-1; ubiquitin; SUMO-1.;

    Sammanfattning : Brutons tyrosine kinase (Btk) is a non-receptor tyrosine kinase related to the Src family of kinases. Mutations in various parts of the gene have been shown to cause X-linked agammaglobulinemia (XLA), a primary immunodeficiency in humans, characterized by a defect in B-cell development. LÄS MER