Sökning: "stable transfection"

Visar resultat 1 - 5 av 16 avhandlingar innehållade orden stable transfection.

  1. 1. Activation and Regulation of TRPV1 : Studies in Recombinant Human Neuroblastoma TRPV1-SHSY5Y Cells

    Författare :Johanna EL Andaloussi-Lilja; Anna Forsby; Antonio Ferrer-Montiel; Stockholms universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; nociception; neurotrophic factors; eye irritation; stable transfection; Ca2 ; Neurochemistry; Neurokemi; Neurochemistry and Molecular Neurobiology; neurokemi med molekylär neurobiologi;

    Sammanfattning : TRPV1 is a transmembrane non-selective cation channel with preference for Ca2+. The receptor is primarily localised on dorsal root ganglion neurons and is activated by numerous endogenous and exogenous potentially irritating ligands eliciting pain. LÄS MER

  3. 2. Expression of recombinant neutralizing anti-HIV-1 antibodies in bacteria and eukaryotic cells

    Författare :Fayezeh Yari; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Phage display; vector; monoclonal antibody; antigen; eukaryotic; prokaryotic; transfection; selection; transient expression; stable cell line; neutralizing antibody; expression; HIVA-1; gp41; gp120;

    Sammanfattning : The main purpose of this study was to investigate and optimize techniques for eukaryotic expression of antibody molecules (IgG, Fab and scFV-ê formats), derived from an immune phage displayed Fab library and reactive against HIV-1 glycoproteins gp120 and gp41. Light and heavy-chain (Fd) DNA from a number of Fab clones were transferred from a prokaryotic phagemid vectors to mammalian expression vectors. LÄS MER

  4. 3. Cell-penetrating peptides; chemical modification, mechanism of uptake and formulation development

    Författare :Kariem Ezzat; Ülo Langel; Steven Dowdy; Stockholms universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; cell penetrating peptides; gene therapy; scavenger receptors; pharmaceutical formulation; solid dispersion; neurokemi med molekylär neurobiologi; Neurochemistry with Molecular Neurobiology;

    Sammanfattning : Gene therapy holds the promise of revolutionizing the way we treat diseases. By using recombinant DNA and oligonucleotides (ONs), gene functions can be restored, altered or silenced according to the therapeutic need. LÄS MER

  5. 4. Linear and Branched Chitosan Oligomers as Delivery Systems for pDNA and siRNA In Vitro and In Vivo

    Författare :Mohamed Mahmoud Issa; Per Artursson; Arto Urtti; Uppsala universitet; []
    Nyckelord :Medical sciences; Chitosan oligomers; gene delivery; gene expression; pDNA; siRNA; MEDICIN OCH VÅRD;

    Sammanfattning : In this thesis, chitosan, a biocompatible polysaccharide that has been approved as a food additive was selected as a platform for the development of safe, efficient non-viral gene delivery systems to mammalian cells. Previously, chitosan-based gene formulations had been generally associated with high molecular weight chitosans, which were poorly characterised in terms of molecular weight distribution and degree of acetylation. LÄS MER

  7. 5. Signaling pathways in Drosophila immunity

    Författare :Shiva Seyedoleslami Esfahani; Ylva Engström; Steven Wasserman; Stockholms universitet; []
    Nyckelord :Drosophila immunity; signaling pathways; Cactus; NFκB; Molecular Biology; molekylärbiologi;

    Sammanfattning : Drosophila relies on innate immunity to protect itself from its hostile environment throughout its life cycle. Despite the remarkable progress in understanding many aspects of Drosophila immunity, there are still big gaps in our knowledge. LÄS MER