Sökning: "solution hybridization"

Visar resultat 1 - 5 av 34 avhandlingar innehållade orden solution hybridization.

  1. 1. Concatemerization of Synthetic Oligonucleotides

    Författare :Lu Sun; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; gel electrophoresis; DNA-binding; QCM-D; DNA concatemer; hybridization; DNA formation; self-assembly;

    Sammanfattning : DNA nanotechnology has become an important research field because of its advantages in high predictability and accuracy of base-paring recognition. For example, the DNA concatemer, one of the simplest DNA constructs in shape, has been used to enhance signals in biosensing. LÄS MER

  3. 2. On Hybrid Fuel Cell and Battery Systems for Maritime Applications

    Författare :Ariel Chiche; Carina Lagergren; Göran Lindbergh; Ivan Stenius; Lars Eriksson; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Fuel Cell; Autonomous Underwater Vehicles AUVs ; Hybridization; Optimization; Buoyancy; Battery; Hydrogen Storage; Oxygen Storage; Dead-End Anode; Purge; Bränslecell; autonom undervattensfarkost AUV ; hybridisering; optimering; flytkraft; batteri; vätgaslagring; syrelagring.; Chemical Engineering; Kemiteknik; Chemical Engineering; Kemiteknik;

    Sammanfattning : Hydrogen is seen as a key player in leading to a sustainable energy sector and decarbonizing the transport sector. Proton Exchange Membrane Fuel Cells (PEMFCs) consume hydrogen and oxygen to generate water, heat and electricity. LÄS MER

  4. 3. The Light-up Probe

    Författare :Nicke Svanvik; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; asymmetric cyanine dyes; light-up probes; PNA; real-time PCR; LightUp Probe; fluorescent hybridization probes; homogeneous hybridization assays;

    Sammanfattning : The work presented in this thesis concerns the development of light-up probes and their potential applications in PCR based molecular diagnostics. The light-up probe is a nucleic acid analog (here PNA) oligomer linked to an asymmetric cyanine dye (here thiazole orange) and it combines the excellent hybridization properties of PNA with the large fluorescence enhancement of the dye upon binding to DNA. LÄS MER

  5. 4. Genotyping RNA and DNA using padlock probes

    Författare :Dan-Oscar Antson; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Padlock probe; enzymatic synthesis; PCR; in situ hybridization; RNA-templated DNA ligation; Genetik; Clinical genetics; Klinisk genetik; Medical Genetics; medicinsk genetik;

    Sammanfattning : Novel techniques are needed to investigate the genetic variation revealed in the first draft of the human genome sequence. Padlock probes are recently developed reagents, suitable for detecting single-nucleotide variations of DNA and RNA in situ or in solution. LÄS MER

  7. 5. Molecular pharmacology of amiodarone

    Författare :Viktor Drvota; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Amiodarone; thyroid hormone receptors; B-adrenergic receptors; AT-1 cardiomyocytes; Western blot; solution hybridization; Fura-2; Langendorff heart; mouse; guinea pig;

    Sammanfattning : Amiodarone (Am) is a potent class III antiarrhythmic drug. Its use is limited due to serious side effects and it was therefore used only when other therapeutic interventions proved to be ineffective. LÄS MER