Sökning: "size exclusion chromatography"
Visar resultat 1 - 5 av 55 avhandlingar innehållade orden size exclusion chromatography.
Sammanfattning : Liquid chromatography is an essential technique in manufacturing biopharmaceuticals where it is used on all scales from analytical applications in R&D to full-scale production. In chromatography the target molecule, typically a protein, is separated and purified from other components and contaminants. LÄS MER
2. Affinity-, Partition- and Permeability Properties of the Human Red Blood Cell Membrane and Biomembrane Models, with Emphasis on the GLUT1 Glucose Transporter
Sammanfattning : The human glucose transporter GLUT1 is abundant in red blood cells, the blood-brain barrier and epithelial cells, where it mediates the transport of the energy metabolite, glucose. In the present work some properties of GLUT1, including affinity binding of both substrates and inhibitors, transport rates as well as permeabilities of aromatic amino acids and drug-membrane interactions were analyzed by chromatographic methods. LÄS MER
Sammanfattning : This thesis details various techniques and considerations for the purification of complex biomacromolecules. Initially an α-mannosidase from babaco fruit was purified using anion exchange-, lectin affinity- and size exclusion chromatography. LÄS MER
Sammanfattning : Antibodies are part of the immune system where their purpose is to recognize and neutralize foreign species such as viruses and bacteria, thus preventing and curing infections. The trend of using antibodies in the treatment of, for example, cancer is increasing. LÄS MER
Sammanfattning : The need for new renewable products has enhanced the interest in cellulose and hemicellulose as raw material. The wood plant cell wall is a complex mixture of cellulose, hemicellulose, lignin and minor amounts of pectin, proteins and extractives. Several interactions between the compounds make them difficult to separate without modification. LÄS MER