Sökning: "size exclusion chromatography"

Visar resultat 1 - 5 av 61 avhandlingar innehållade orden size exclusion chromatography.

  1. 1. Characterizing Chromatography Media : NMR-based Approaches

    Författare :Fredrik Elwinger; István Furó; Stéphane Viel; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; chromatography; nuclear magnetic resonance; NMR; self-diffusion; magic-angle spinning; quantitative; distribution coefficient; pore size; Kemi; Chemistry;

    Sammanfattning : Liquid chromatography is an essential technique in manufacturing biopharmaceuticals where it is used on all scales from analytical applications in R&D to full-scale production. In chromatography the target molecule, typically a protein, is separated and purified from other components and contaminants. LÄS MER

  3. 2. Affinity-, Partition- and Permeability Properties of the Human Red Blood Cell Membrane and Biomembrane Models, with Emphasis on the GLUT1 Glucose Transporter

    Författare :Christine Lagerquist Hägglund; Per Lundahl; Jørgen Vinten; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biochemistry; Affinity; Aromatic amino acids; Binding; Biomembrane; Biotin; Chromatography; Cytochalasin B; Dihydrocytochalasin B; Dissociation constant; Drug absorption; Ethanol; Equilibrium; Glucose; GLUT1; Immobilization; Immobilized biomembrane affinity chromatography; Immobilized liposome chromatography; Interaction; Liposome; Membrane protein; Membrane vesicle; Partitioning; Phospholipid bilayer; Proteoliposome; Quantitative; Red blood cell; Specific; Streptavidin; Tyrosine; Tryptophan; Biokemi; Biochemistry; Biokemi;

    Sammanfattning : The human glucose transporter GLUT1 is abundant in red blood cells, the blood-brain barrier and epithelial cells, where it mediates the transport of the energy metabolite, glucose. In the present work some properties of GLUT1, including affinity binding of both substrates and inhibitors, transport rates as well as permeabilities of aromatic amino acids and drug-membrane interactions were analyzed by chromatographic methods. LÄS MER

  4. 3. Purification Processes for Complex Biomacromolecules

    Författare :Hans Blom; Helena Grennberg; Mats Lundgren; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Chromatography; enzymes; mucins; fractionation; plasmid DNA; analysis; clarification; process design; Chemistry with specialization in Surface Biotechnology; Kemi med inriktning mot ytbioteknik;

    Sammanfattning : This thesis details various techniques and considerations for the purification of complex biomacromolecules. Initially an α-mannosidase from babaco fruit was purified using anion exchange-, lectin affinity- and size exclusion chromatography. LÄS MER

  5. 4. Modeling Antibody Aggregation in Downstream Processing

    Författare :Frida Ojala; Avdelningen för kemiteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Antibody; Aggregation; Downstream processing; Size exclusion chromatography; Reversible reaction model; General rate model;

    Sammanfattning : Antibodies are part of the immune system where their purpose is to recognize and neutralize foreign species such as viruses and bacteria, thus preventing and curing infections. The trend of using antibodies in the treatment of, for example, cancer is increasing. LÄS MER

  7. 5. Isolation, characterization and enzymatic hydrolysis of water-soluble wood polysaccharides

    Författare :Jon Lundqvist; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; size exclusion chromatography; beta-mannosidase; beta-mannanase; endoglucanase; ethyl hydoxyethyl cellulose; metabolism; Biokemi; Biochemistry; Metabolism; microwave irradiation; O-acetyl-galactoglucomannan; isolation;

    Sammanfattning : The need for new renewable products has enhanced the interest in cellulose and hemicellulose as raw material. The wood plant cell wall is a complex mixture of cellulose, hemicellulose, lignin and minor amounts of pectin, proteins and extractives. Several interactions between the compounds make them difficult to separate without modification. LÄS MER