Sökning: "site-directed labeling"

Visar resultat 1 - 5 av 10 avhandlingar innehållade orden site-directed labeling.

1. 1. Lipase-Lipid Interactions Studied by Site-Directed Labeling and Biological Spectroscopy

   Författare :Eva Hedin; Karl Hult; Marek Brzozowski; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; thermomyces lanuginous lipase; site-directed labeling; lipase-lipid interactions; Biochemistry; Biokemi;

   Sammanfattning : This thesis describes the study of lipase–lipid interactions of the triglyceride lipase Thermomyces lanuginosus lipase, TLL, by use of site-directed chemical labeling and biological spectroscopy. Several single-cysteine variants of TLL were produced, and labeled with probes containing either an affinity-, a fluorescence- or a spin-label functionality. LÄS MER

3. 2. Studies of Local Interactions between and within Proteins using Site-Directed Labeling Techniques

   Författare :Rikard Owenius; Lawrence J. Berliner; Linköpings universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

   Sammanfattning : Proteins are essential participants in virtually all cellular processes. The key to the understanding of the function of a certain protein is a detailed knowledge about its atomic three-dimensional structure. LÄS MER

4. 3. The Interaction Between the Chaperonin GroEL and Human Carbonic Anhydrase II : Protein Folding and Stability Studies

   Författare :Malin Persson; Linköpings universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; carbonic anhydrase; protein folding; protein stability; chaperone; GroEL; folding mechanism; site-directed spinlabeling; electron paramagnetic resonance EPR ;

   Sammanfattning : The presence of GroEL/ES during the refolding of human carbonic anhydrase II was found to increase the yield of active enzyme from approx. 70 to approx. 100%. This chaperone action on the enzyme could be obtained by adding GroEL alone, and the time course in that case was only moderately slower than the spontaneous process. LÄS MER

5. 4. Molecular probes as protein analysis tools in biotechnology

   Författare :Anngelica Jarl; Linköpings universitet; []
   Nyckelord :NATURAL SCIENCES; NATURVETENSKAP;

   Sammanfattning : Protein-labeling today is a work of art, in vivo studies of proteins or other molecules can easily be performed, and the movement of the labeled molecule can be followed in real time. Labeling in vitro gives enormous amount of data in labs all over the world on a daily basis, where protein-protein, protein-DNA or other interactions are studied. LÄS MER

7. 5. Protein engineering for biophysical studies of protein folding, stability and surface interactions

   Författare :Martin Karlsson; Mikael Oliveberg; Linköpings universitet; []
   Nyckelord :Biochemistry; protein folding; NATURAL SCIENCES; NATURVETENSKAP;

   Sammanfattning : The subject of this thesis can be split into two parts, one that is dealing with the stability and stabilization of proteins on its own merits and a second part that deals with the protein adsorption orientation on solid surfaces and how the stability of a protein influences the behavior at a solid/liquid interface. Thus, the common denominator and focus have been on protein stability. LÄS MER