Sökning: "single-chain antibody"

Visar resultat 1 - 5 av 25 avhandlingar innehållade orden single-chain antibody.

1. 1. Construction, expression and evaluation of anti-keratin 8 single-chain antibody fragments

   Författare :Rozbeh Jafari; Birgitta Sundström; Jörgen Carlsson; Karlstads universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Single-chain antibody fragments; keratin 8; site-directed mutagenesis; heterologous expression; P. pastoris; design of experiments; multicellular tumor spheroids; immunotargeting; Biomedical Sciences; Biomedicinsk vetenskap;

   Sammanfattning : Antibodies are glycoproteins specifically binding to a variety of antigens and today extensively used in prevention, diagnosis and treatment of diseases. Carcinomas originate from epithelial tissues and are the most common forms of human malignancies. In the necrotic areas of carcinomas significant amounts of keratins (K) are found extracellularly. LÄS MER

3. 2. Functional mapping and in vivo metabolism of the monoclonal antibody TS1 and its single-chain fragment : Its interaction with the antigen and the anti-idiotype

   Författare :Patrik Holm; Birgitta Sundström; Lars Gedda; Karlstads universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; single-chain antibody; functional mapping; site-directed mutagenesis; immune complex; metabolism; Molecular biology; Molekylärbiologi; Biomedical Laboratory Science; Biomedicinsk laboratorievetenskap;

   Sammanfattning : Antibodies are proteins capable of specific interactions to a wide range of molecules. These interactions are facilitated by the complementary determining regions (CDR).Carcinomas are the most common of human cancers and they release significant amount of cytokeratins (CK) in the necrotic areas of the tumors. LÄS MER

4. 3. Single-chain antibody construction and functional mapping of the monoclonal antibody TS1 : Its interaction with the antigen and the anti-idiotype

   Författare :Patrik Holm; Birgitta Sundström; Per-Åke Nygren; Karlstads universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Chemistry; Kemi; Chemistry; Kemi; Biomedicin; Biomedical Laboratory Science;

   Sammanfattning : The aims of this study are to synthesize and produce a single-chain antibody (scFv) of the anti-cytokeratin 8 monoclonal IgG antibody TS1 and to functionally map amino acid residues important for the interaction with its antigen and the anti-idiotypic antibody TS1. The TS1 antibody has been shown to be effective in binding cytokeratin 8 (CK8) expressed in tumors in vivo and is proposed to be useful in immunotargeting and/or immunotherapy. LÄS MER

5. 4. Retargeting of adenovirus vectors. Viral ligands and cellular receptors

   Författare :Petra Henning; Göteborgs universitet; []
   Nyckelord :Adenovirus; vector; gene therapy; gene transfer; retargeting; detargeting; tumor targeting; fiber; pIX; affibody molecule; single chain antibody;

   Sammanfattning : Gene therapy is one of the newest technologies attempted to treat human cancer. Progress in the field has however been hampered by the lack of efficient and safe vectors. Ideal vectors should specifically transduce desired cells in vivo. This thesis is devoted to the development and evaluation of retargeted adenovirus type 5 (Ad5) vectors. LÄS MER

7. 5. Genetic re-targeting and de-targeting of adenovirus type 5 in order to create vectors for gene therapy

   Författare :Susanna Myhre; Göteborgs universitet; []
   Nyckelord :Adenovirus; gene therapy; vector; re-targeting; de-targeting; fiber; pIX; affibody molecule; single chain antibody;

   Sammanfattning : Gene therapy has been considered to be a revolutionary development in medicine, whereby the cause and not the symptoms of the disease would be treated. These expectations have as yet not been realized, mainly due to lack of suitable vectors. LÄS MER