Sökning: "single-cell monitoring"

Visar resultat 1 - 5 av 23 avhandlingar innehållade orden single-cell monitoring.

  1. 1. Electrochemical monitoring of living cells

    Författare :Christer Spegel; Centrum för analys och syntes; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; PC12; redox; electrochemistry; NAD P H; Single cell; Microchip; S. cerevisiae;

    Sammanfattning : This thesis presents the utilization of electroanalytical methods for analysis of dynamic cellular processes such as exocytosis, intracellular redox reactions and cell surface interactions. Microchips have been designed and manufactured with the purpose of monitoring dopamine exocytosis from single or a small ensemble of PC12 cells. LÄS MER

  3. 2. iLocks: a novel tool for RNA assays with improved specificity

    Författare :Tomasz Krzywkowski; Mats Nilsson; Gunnar von Heijne; Weng-Onn Lui; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; RNA; miRNA; non-coding RNA; padlock probes; rolling circle amplification; invader; single cell; in situ; adenovirus; virology; diagnostics; Biochemistry; biokemi;

    Sammanfattning : The Central Dogma of molecular biology describes a framework for how genetic information is transferred in cells, placing RNA as a messenger between DNA and translated proteins. During the last years, interest in RNA research has grown tremendously due to the increasing understanding and recognition of the importance of RNA in regulation of gene expression, biochemical catalysis, and genome integrity surveillance. LÄS MER

  4. 3. An NADH-Coupled Biosensor for Engineering Redox Metabolism in Saccharomyces cerevisiae

    Författare :Jan Knudsen; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Yeast; redox balance; glycerol whole-cell biocatalysis; single-cell monitoring; FACS;

    Sammanfattning : Baker’s yeast, Saccharomyces cerevisiae’s potential in industrial biotechnology for producing valuable products e.g. biofuels, bulk chemicals, bio-flavours and pharmaceuticals is established. LÄS MER

  5. 4. Single cell studies of human T cell function with applications for HIV-1

    Författare :Johanna Tauriainen; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : T cells are one of the body’s main defenses against viruses and cancers and are therefore considered to play a major role in immunotherapies after stem cell transplantation and in HIV-1 vaccine and cure strategies. However, malignancies and chronic viral infections, such as HIV-1, eventually cause the T cells to become dysfunctional, resulting in a loss of control. LÄS MER

  7. 5. Single cell carbon and nitrogen dynamics in chain forming diatoms, including their resting stage

    Författare :Rickard Stenow; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Diatoms; Resting stages; N cycling; Chain formation; Secondary ion mass spectrometry SIMS ;

    Sammanfattning : The oceans are a fundamental part of all life on earth, accounting for more than half of Earth’s oxygen production. The ocean is also key to long term carbon dioxide sequestration. Diatoms are a group of phytoplankton differentiated by their silica shell/frustule and account for ~20% of global primary production. LÄS MER