Sökning: "single-cell RNA seq"

Visar resultat 1 - 5 av 40 avhandlingar innehållade orden single-cell RNA seq.

1. 1. Computational methods for analysis and visualization of spatially resolved transcriptomes

   Författare :Jose Fernandez Navarro; Joakim Lundeberg; Martin Hemberg; KTH; []
   Nyckelord :RNA; RNA-seq; single cell; scRNA-seq; transcriptomics; spatial transcriptomics; brain; 3D; Alzheimer’s disease; Bioteknologi; Biotechnology;

   Sammanfattning : Characterizing the expression level of genes (transcriptome) in cells and tis- sues is essential for understanding the biological processes of multicellular or- ganisms. RNA sequencing (RNA-seq) has gained traction in the last decade as a powerful tool that provides an accurate quantitative representation of the transcriptome in tissues. LÄS MER

3. 2. Utilization of single-cell RNA-Seq and genome-scale modeling for investigating cancer metabolism

   Författare :Johan Gustafsson; Chalmers tekniska högskola; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; genome-scale metabolic modeling; cancer; metabolism; single-cell RNA-Seq;

   Sammanfattning : Cancer remains a leading cause of death worldwide, and its dysregulated metabolism is a promising target for therapy. However, metabolism is complex to study – the metabolism of a cell involves the interplay of thousands of chemical reactions that are combined in different ways across tissues and cell types. LÄS MER

4. 3. Computational Single-Cell Genomics Methods for Cell State Estimation and their Application to Hematopoietic Systems

   Författare :Parashar Dhapola; Avdelningen för molekylär hematologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Hematopoietic Stem Cell; Single-Cell; Genomics; Epigenomics; transcriptomics; bioinformatics;

   Sammanfattning : The focus of this doctoral dissertation was to develop novel and scalable computational genomics methods for analysing single-cell genomic modalities. Scarf, a highly memory-efficient single-cell data analysis toolkit, was developed to enable an analysis of very large-scale datasets on personal computers, and the execution of parallel workflows on server-scale computational systems. LÄS MER

5. 4. Progression of RNA-sequencing to single-cell applications

   Författare :Daniel Ramsköld; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : New methods enable new discoveries. My time as a PhD student has run in parallel with the maturation of the RNA-seq method, and I have used it to discover basic properties of gene expression and transcriptomes. My part has been bioinformatics – the computer analysis of biological data. LÄS MER

7. 5. Host-parasite interactions in space and time

   Författare :Franziska Hildebrandt; Johan Ankarklev; Volker T. Heussler; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Malaria; liver-stage infection; Toxoplasma; host-parasite interactions; spatial transcriptomics; single-cell omics; single-cell Dual-seq; molekylär biovetenskap; Molecular Bioscience;

   Sammanfattning : Unicellular parasites of the apicomplexan phylum have a considerable effect on global health and agriculture. Two prominent examples of this phylum include malaria causing parasites of the Plasmodium genus and the widely prevalent parasite Toxoplasma gondii. LÄS MER