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1. 1. Making Visible the Proximity Between Proteins

   Författare :Carl-Magnus Clausson; Ola Söderberg; Jens Coorssen; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Proximity ligation assay; In situ PLA; rolling circle amplification; protein interaction; protein-protein interaction; in situ; single cell; single molecule; protein complex; antibody; cancer; tissue section; microscopy; image analysis; system biology; multiplex; dynamic range; methods development; systems biology; Molekylär medicin; Molecular Medicine;

   Sammanfattning : Genomic DNA is the template of life - the entity which is characterized by a self-sustaining anatomical development, regulated signaling processes, the ability to reproduce and to respond to stimuli. Through what is classically known as the central dogma, the genome is transcribed into mRNA, which in turn is translated into proteins. LÄS MER

3. 2. Unravelling protein stability and aggregation

   Författare :Tanja Weiffert; NanoLund: Centre for Nanoscience; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; protein stability; protein aggregation; amyloid beta peptide; protein stabilization; split GFP; monellin; secretagogin; ; aggregation kinetics; toxicity; aggregation mechanism; coaggregation;

   Sammanfattning : Proteins are complex macromolecules that are fundamental to all living species. The stability of proteins are governed by non-covalent forces within the protein and between the protein and its surrounding environment. LÄS MER

4. 3. Statistical processing of Flash X-ray Imaging of protein complexes

   Författare :Alberto Pietrini; Carl Nettelblad; Johan Bielecki; Janos Hajdu; Flavio Capotondi; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; X-ray; XFEL; single particle; hit-finder; statistical hit-finder; single protein; protein complex; RNA polymerase II; FEL; free-electron laser; FXI; Flash X-ray Imaging; CXI; CSPAD; x-ray imaging; COACS; Physics with specialization in Biophysics; Fysik med inriktning mot biofysik;

   Sammanfattning : Flash X-ray Imaging (FXI) at X-ray Free Electron Lasers (XFELs) is a promising technique that permits the investigation of the 3D structure of molecules without the need for crystallization, by diffracting on single individual sample particles.In the past few years, some success has been achieved by using FXI on quite large biological complexes (40 nm-1 μm in diameter size). LÄS MER

5. 4. Recombinant subunit vaccines : protein immunogens

   Författare :Sissela Liljeqvist; KTH; []
   Nyckelord :;

   Sammanfattning : Recombinant techniques provide valuable tools for thedevelopment of modern subunit vaccines. In this thesisdifferent systems for production of recombinant subunitvaccines are presented. The strategies investigated includeprotein immunogens, live bacterial vectors and nucleicacids. LÄS MER

7. 5. Exploring small heat shock protein chaperones by crosslinking mass spectrometry

   Författare :Wietske Lambert; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; cross-linking; mass spectrometry; protein-protein interactions; Hsp21; BS3; DTSSP; MALDI-TOF TOF; client protein; substrate; aggregation; unfolded protein; chaperone; Small heat shock protein; crosslinking;

   Sammanfattning : Together with other molecular chaperones, small heat shock proteins are key components of the protein quality control system, which is comprised of several hundred proteins and acts to maintain proteome homeostasis in the cell. Small heat shock proteins bind unfolding proteins at an early stage, to prevent these from further unfolding and aggregating. LÄS MER