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Visar resultat 1 - 5 av 1103 avhandlingar som matchar ovanstående sökkriterier.

1. 1. Into the Single-Verse : Subtle gene expression differences between virtually identical single cells are informative of gene regulation

   Författare :Marcel Tarbier; Marc Friedländer; Nils Blüthgen; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Single-cell; quantitative; RNA biology; miRNA; functional genomics; molekylär biovetenskap; Molecular Bioscience;

   Sammanfattning : The ability to profile transcriptomes and proteomes in a high-throughput fashion in single cells has truly revolutionized functional genomics, and countless functional and regulatory insights have been based on these technologies. While major applications include the discovery of new cell types and the a posteriori sorting of cell populations, studies of gene expression noise and gene co-expression have made use of this inter-cellular heterogeneity in a genuine quantitative fashion. LÄS MER

3. 2. Detection and Sequencing of Amplified Single Molecules

   Författare :Rongqin Ke; Mats Nilsson; Ulf Landegren; Radoje Drmanac; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; padlock probes; proximity ligation assay; rolling circle amplification; circle-to-circle amplification; single molecule detection; amplified single molecules; sequencing; in situ; single cell; Molecular Biotechnology; Molekylär bioteknik;

   Sammanfattning : Improved analytical methods provide new opportunities for both biological research and medical applications. This thesis describes several novel molecular techniques for nucleic acid and protein analysis based on detection or sequencing of amplified single molecules (ASMs). LÄS MER

4. 3. Genotyping and Mutation Detection In Situ : Development and application of single-molecule techniques

   Författare :Ida Grundberg; Mats Nilsson; Ulf Landegren; Olli-Pekka Kallioniemi; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; padlock probes; in situ; rolling circle amplification; mRNA; genotyping; mutation detection; cancer; tissue sections; diagnostics; single-molecule; single-cell; microscopy; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Molekylär medicin; Molecular Medicine;

   Sammanfattning : The human body is composed of trillions of cells closely working together to maintain a functional organism. Every cell is unique in molecular composition and can acquire genetic variations that might cause it to turn pathological. LÄS MER

5. 4. Single molecule dynamics

   Författare :Lars Edman; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Properties found in an ensemble of molecules may not be understood if it is not possible to study each molecule one by one. This thesis is concerned with the study of the dynamic properties of individual complex biological molecules as observed by confocal single-molecule fluorescence spectroscopy. LÄS MER

7. 5. Control Strategy for Energy Efficient Fluid Power Actuators : Utilizing Individual Metering

   Författare :Björn Eriksson; Jan-Olov Palmberg; Jonas Larsson; Jochen Pohl; Linköpings universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Fluid power; mode switch; energy efficient; independent metering; split spool; poppet; Fluid mechanics; Strömningsmekanik;

   Sammanfattning : This thesis presents a solution enabling lower losses in hydraulic actuator systems. A mobile fluid power system often contains several different actuators supplied with a single load sensing pump. One of the main advantages is the need of only one system pump. This makes the fluid power system compact and cost-effective. LÄS MER