Sökning: "single cell"

Visar resultat 16 - 20 av 1822 avhandlingar innehållade orden single cell.

  1. 16. High Content Analysis of Proteins and Protein Interactions by Proximity Ligation

    Författare :Karl-Johan Leuchowius; Ola Söderberg; Ulf Landegren; Paul Goldsmith; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; in situ; proximity ligation; flow cytometry; high content screening; rolling circle amplification; in situ PLA; single-cell; single-molecule; protein interactions; drug screening; post-translational modifications; Molecular medicine; Molekylär medicin; Molekylär medicin; Molecular Medicine;

    Sammanfattning : Fundamental to all biological processes is the interplay between biomolecules such as proteins and nucleic acids. Studies of interactions should therefore be more informative than mere detection of expressed proteins. Preferably, such studies should be performed in material that is as biologically and clinically relevant as possible, i.e. LÄS MER

  2. 17. Pulsatile insulin release from single islets of Langerhans

    Författare :Johanna Westerlund; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Cell biology; islets of Langerhans; insulin; ELISA; cytoplasmic calcium; oscillations; type 2 diabetes; glucose; diazoxide; potassium; tolbutamide; DNP; antimycin A; iodoacetamide; microfluorometry; fura-2; KATP channels; Cellbiologi; Cell biology; Cellbiologi; medicinsk cellbiologi; Medical Cell Biology;

    Sammanfattning : Insulin release from single islets of Langerhans is pulsatile. The secretory activities of the islets in the pancreas are coordinated resulting in plasma insulin oscillations. Nutrients amplitude-regulate the insulin pulses without influencing their frequency. LÄS MER

  3. 18. Miniaturised Microwell-based Cell Assays

    Författare :Emilie Weibull; Helene Andersson Svahn; Albert van den Berg; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; microwell; miniaturisation; high-resolution imaging; high-throughput; cell culture; single-cell; clone; heterogeneity; antibiotic susceptibility testing; concentration gradient; interfacing; microchannels; cell retrieval;

    Sammanfattning : Cell heterogeneity in genetically identical cell populations is becoming a well-known and important phenomenon in cell biology. Current methods commonly utilise population-based analysis founded on averaged result. Hence there is a need for high-throughput cell assays on the single-cell level. LÄS MER

  4. 19. Exploring Cellular Dynamics : From Vesicle Tethering to Cell Migration

    Författare :Parham Ashrafzadeh; Johan Kreuger; Jonas Fuxe; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; angiogenesis; cancer; cell migration; exocyst complex; exocytosis; FGD5; lipid rafts; plasma membrane; receptor trafficking; VEGFR2;

    Sammanfattning : Cells in the body communicate with each other in order to cooperate efficiently. This communication is in part achieved by regulated secretion of signaling molecules, which when released from a cell may activate receptors present at the plasma membrane of an adjacent cell. Such signals affect both cell fate and behavior. LÄS MER

  5. 20. Regulatory programs controlling profileration during Drosophila nervous system development

    Författare :Erika Gunnar; Stefan Thor; Jan-Ingvar Jönsson; Udo Häcker; Linköpings universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES;

    Sammanfattning : The central nervous system (CNS) is the most complex organ in the body, responsible for complex functions, including thinking, reasoning and memory. The CNS contains cells of many different types, often generated in vast numbers. LÄS MER