Sökning: "silencing"

Visar resultat 1 - 5 av 267 avhandlingar innehållade ordet silencing.

  1. 1. Silencing and DNA double-strand break repair in budding yeast

    Författare :Andreas Kegel; Stefan Åström; Stephan Marcand; Stockholms universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Silencing; Reb1; DSB; NHEJ; Nej1; illegitimate recombination; yeast; Developmental biology; Utvecklingsbiologi;

    Sammanfattning : Transcriptional silencing that makes large chromosomal domains inaccessible for the transcriptional apparatus is nucleated at DNA elements called silencers. In K.lactis a 102bp HMLα silencer was defined revealing three distinct protein-binding regions (A, B, and C) that were required for silencing of HMLα. LÄS MER

  2. 2. Mechanisms of Transgene Silencing in Neural Cells -Implications for Ex Vivo Gene Therapy to the Brain

    Författare :Nina Rosenqvist; Neurobiologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; neurophysiology; Neurologi; Neurology; neuropsychology; ChIP; rat; transgene; insulator; differentiation; GFP; lentivirus; DNA methylation; histone deacetylation; chromatin; expression; silencing; stem cell; progenitor; gene therapy; neurofysiologi; neuropsykologi; CNS; transplantation;

    Sammanfattning : The use of genetically modifi ed neural stem and progenitor cells could possibly be a future strategy for repairing a brain suffering from neurodegeneration. However, so far it has proven diffi cult to maintain sufficient levels of a transferred gene in suitable cell types, after transplantation. LÄS MER

  3. 3. Mating type switching and transcriptional silencing in Kluyveromyces lactis

    Författare :Emad Barsoum; Åström Stefan; Laura Rusche; Stockholms universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Mating type switch; transcriptional silencing; alpha3; transposase; Ume6; Mts1; Ras; cAMP; K. lactis; Developmental biology; Utvecklingsbiologi; utvecklingsbiologi; Developmental Biology;

    Sammanfattning : To explore the similarities and differences of regulatory circuits among budding yeasts, we characterized the role of unscheduled meiotic gene expression 6 (UME6) and a novel mating type switching pathway in Kluyveromyces lactis. We found that Ume6 was required for transcriptional silencing of the cryptic mating-type loci HMLα and HMRa. LÄS MER

  4. 4. Chromatin Dynamics in the Fission Yeast, Schizosaccharomyces pombe

    Författare :Carolina Kristell; Pernilla Bjerling; Per Sunnerhagen; Uppsala universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Chromatin; Gene induction; Silencing; Subnuclear localization; Stress; Molecular biology; Molekylärbiologi; Molekylär genetik; Molecular Genetics;

    Sammanfattning : In the eukaryotic cell nucleus, spatial organization and dynamics of the genome is important in the regulation of gene expression. This thesis describes the use of the fission yeast, Schizosaccharomyces pombe, to study chromatin regulation and dynamics. We used nitrogen starvation to induce transcription of genes in fission yeast cells. LÄS MER

  5. 5. Molecular Insights into Kcnq1ot1 Noncoding Antisense RNA Mediated Long Range Transcriptional Gene Silencing

    Författare :Radha Raman Pandey; Chandrasekhar Kanduri; Karl Ekwall; Uppsala universitet; []
    Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; epigenetics; gene silencing; noncoding RNA; genomic imprinting; Kcnq1ot1; Medical genetics; Medicinsk genetik;

    Sammanfattning : Non-coding antisense RNAs have been implicated in the epigenetic silencing of individual gene as well as chromosomal domains. While silencing of the overlapping gene by antisense RNAs has been well investigated, their functional role in silencing of chromosomal domains remains enigmatic. LÄS MER