Sökning: "sheathless"

Hittade 5 avhandlingar innehållade ordet sheathless.

1. 1. Development of Sheathless Electrospray Mass Spectrometry and Investigations of Associated Electrochemical Processes – A Fairy Tale

   Författare :Stefan Nilsson; Karin Markides; Leif Nyholm; Jonas Bergquist; Per Andrén; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; electrospray; sheathless; mass spectrometry; capillary electrophoresis; electrochemistry; chromatography; interface; fairy dust; gold; Analytisk kemi; Analytical chemistry; Analytisk kemi;

   Sammanfattning : In microscale separations, such as capillary electrophoresis and -liquid chromatography, the liquid flow rates are in the order of nanoliters per second. If such flow rates are to be interfaced with a mass spectrometer (MS) using electrospray (ES) ionization, without loss of separation efficiency, each fraction of the analyte zone must remain undisturbed by the high voltage contact necessary for ES. LÄS MER

3. 2. Electrifying the Molecules of Life : Peptide and Protein Analysis by Capillary Electrophoresis Coupled to Electrospray Ionization Mass Spectrometry

   Författare :Magnus Wetterhall; Jonas Bergquist; Karin E. Markides; Dominic M. Desiderio; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; Capillary electrophoresis CE ; Electrospray ionization ESI ; Mass spectrometry MS ; Peptides; Proteins; Cerebrospinal fluid CSF ; Graphite coating; Monomer coating; Polymer coating; Analytisk kemi; Analytical chemistry; Analytisk kemi;

   Sammanfattning : This thesis describes the current status and novel aspects of the analysis of the molecules of life, i.e. peptides and proteins, using capillary electrophoresis (CE) coupled to mass spectrometry (MS) via (sheathless) electrospray ionization (ESI). Early reports of sheathless CE-ESI-MS were plagued by limited lifetimes of the electrospray emitter. LÄS MER

4. 3. Microstructures for Chemical Analysis : Design, Fabrication and Characterisation

   Författare :Malin Svedberg; Fredrik Nikolajeff; Greger Thornell; Jan-Åke Schweitz; Sami Franssila; Uppsala universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Materials science; electrospray ionisation mass spectrometry; polymer microfabrication; paraffin actuators; hot embossing; Materialvetenskap; Materials science; Teknisk materialvetenskap;

   Sammanfattning : The interest for miniaturisation in chemical and biological analysis has increased in recent years. In this work, the design, fabrication and characterisation of tools for microanalysis have been studied. The focus is set on polymer microchips for applications in chemical analysis. LÄS MER

5. 4. Development of Micro Liquid Separation Techniques using Electrospray Ionisation Mass Spectrometry in the Analysis of Polar Compounds and Proteins/Peptides

   Författare :Jenny Samskog; Elsa Lundanes; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; Analytisk kemi; Analytical chemistry; Analytisk kemi; Analytical Chemistry; Analytisk kemi;

   Sammanfattning : Electrospray ionisation (ESI) coupled to mass spectrometry (MS) is one of the most important detection techniques for chemical analysis of small drugs as well as large biomolecules in life science today. In this thesis, aspects on improved compatibility between liquid based separation systems and mass spectrometric detection were investigated regarding buffers, sample preparation and analysis of polar compounds as well as peptides and protein digests for enhanced ESI-MS performance. LÄS MER

7. 5. Acoustic Forces in Cytometry and Biomedical Applications: Multidimensional Acoustophoresis

   Författare :Carl Grenvall; Avdelningen för Biomedicinsk teknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Acoustophoresis; Microfluidics; Ultrasound; Multinode; Cell Sorting; Particle Positioning; Cytometry; Lab-on-a-Chip; µTAS; Binary Separation; Acoustic Barriers; Acoustic Particle Manipulation; Acoustic Standing Waves; Blood Separation; Milk Analysis;

   Sammanfattning : Over the last decades the ongoing work in the fields of Lab-on-a-Chip and Micro-Total-Analysis-Systems has led to the discovery of new or improved ways to handle and analyse small volumes of biofluids and complex biosuspensions. The benefits of working on the microscale include: miniaturization of the analysis systems with less need for large sample volumes; temporal and spatial control of suspended particle/cell positions; low volume sheath flow lamination or mixing; novel separation techniques by using forces inherent to the microscale domain; precise regulation of sample temperatures and rapid analysis with less volumes needed to be processed. LÄS MER