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Visar resultat 1 - 5 av 1411 avhandlingar som matchar ovanstående sökkriterier.

1. 1. Sequencing cancer

   Författare :Una Kjällquist; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Cancer forms highly heterogeneous tissues at several molecular levels, genomic, proteomic, transcriptomic and other epigenetic traits. The level of complexity is further augmented by the dynamic nature of tumor progression with cancer cell populations evolving in a clonal manner. LÄS MER

3. 2. On Transcriptome Sequencing

   Författare :Daniel Klevebring; Joakim Lundeberg; Ulf Gyllensten; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Transcriptome; RNA-seq; DNA sequencing; gene expression profiling; non-coding RNA; small RNA; Functional genomics; Funktionsgenomik; Bioinformatics; Bioinformatik; Genetics; Genetik; Molecular biology; Molekylärbiologi; Cell biology; Cellbiologi;

   Sammanfattning : This thesis is about the use of massive DNA sequencing to investigate the transcriptome. During recent decades, several studies have made it clear that the transcriptome comprises a more complex set of biochemical machinery than was previously believed. LÄS MER

4. 3. Targeted Long-read Sequencing : Development and Applications in Medical Genetics

   Författare :Ida Höijer; Ulf B. Gyllensten; Adam Ameur; Lars Feuk; Winston Timp; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; long-read sequencing; targeted sequencing; targeted enrichment; CRISPR-Cas9; off-targets; medical genetics; repeat expansion; structural variant;

   Sammanfattning : Targeted sequencing has the advantage of providing pinpointed DNA information, while costs and data-analysis efforts are reduced. If targeted sequencing is combined with single molecule long-read sequencing, it can become a powerful tool to investigate genomic regions traditionally difficult using the predominantly used short-read sequencing platforms, including repetitive regions and large structural variants. LÄS MER

5. 4. Library Preparation for High Throughput DNA Sequencing

   Författare :Anders Jemt; Joakim Lundeberg; Petri Auvinen; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; DNA; RNA; sequencing; massively parallel sequencing; library preparation; automation; genome; transcriptome; Bioteknologi; Biotechnology;

   Sammanfattning : Order 3 billion base pairs of DNA in the correct order and you get the blueprint of a human, the genome. Before the introduction of massively parallel sequencing a little more than a decade ago it would cost around $10 million to get this blueprint. LÄS MER

7. 5. Tagging systems for sequencing large cohorts

   Författare :Mårten Neiman; Afshin Ahmadian; Peter Savolainen; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; next generation sequencing; genotyping; massive parallel sequencing; 454; Pyrosequencing; amplicon sequencing; enrichment; DNA barcodes; Genetics; Genetik;

   Sammanfattning : Advances in sequencing technologies constantly improves the throughput andaccuracy of sequencing instruments. Together with this development comes newdemands and opportunities to fully take advantage of the massive amounts of dataproduced within a sequence run. LÄS MER