Sökning: "sequence specific primers"

Visar resultat 1 - 5 av 29 avhandlingar innehållade orden sequence specific primers.

  1. 1. Phylogenetic Inference and Allopolyploid Speciation - A Study of Silene section Physolychnis

    Detta är en avhandling från Göteborg : University of Gothenburg

    Författare :Anna Petri; Göteborgs universitet.; Gothenburg University.; [2012]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; allopolyploidization; next-generation sequencing; Physolychnis; sequence specific primers; Silene; species networks; transcriptomics;

    Sammanfattning : The major theme of this thesis is allopolyploidization, with focus on Silene section Physolychnis. The evolutionary history of an allopolyploid species can be established by inferring the sister relationships of the homoeologous sequences from low copy nuclear markers. LÄS MER

  2. 2. Selection of antigens for antibody-based proteomics

    Detta är en avhandling från Stockholm : KTH

    Författare :Lisa Berglund; KTH.; [2008]
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; epitope; antigen; antibody; affinity; protein; proteome; proteomics; bioinformatics; prediction; primer design; sequence similarity; TECHNOLOGY Bioengineering; TEKNIKVETENSKAP Bioteknik;

    Sammanfattning : The human genome is predicted to contain ~20,500 protein-coding genes. The encoded proteins are the key players in the body, but the functions and localizations of most proteins are still unknown. LÄS MER

  3. 3. Arrayed identification of DNA signatures

    Detta är en avhandling från Stockholm : KTH

    Författare :Max Käller; KTH.; [2005]
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; apyrase; allele-specific extension; competitive hybridization; DNA sequencing; genotyping; human papillomavirus HPV ; MC1R; microarray; mutation; p53; protease; TECHNOLOGY Bioengineering; TEKNIKVETENSKAP Bioteknik;

    Sammanfattning : In this thesis techniques are presented that aim to determine individual DNA signatures by controlled synthesis of nucleic acid multimers. Allele-specific extension reactions with an improved specificity were applied for several genomic purposes. Since DNA polymerases extend some mismatched 3’-end primers, an improved specificity is a concern. LÄS MER

  4. 4. Fatty Acyl Reductases and Fatty Acyl Desaturases. Deciphering the biosynthesis of species-specific moth female sex pheromones from common fatty acids

    Detta är en avhandling från Lund University, Dept. of Biology

    Författare :Åsa Hagström; [2013]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Moth; pheromone; insect; evolution; fatty acyl desaturase; fatty acyl reductase; endoplasmic reticulum; fatty acid biosynthesis; GFP; heterologous expression; phylogeny; cell factory; lepidoptera;

    Sammanfattning : Moths (Lepidoptera) are dependent on female produced pheromones, chemical signals, for attracting a mate. Pheromones are most commonly made up by saturated and/or unsaturated fatty acid derived alcohols, acetates, and/or aldehydes that are produced by a set of enzymes in the female pheromone gland. LÄS MER

  5. 5. The use of molecular techniques for identification of genetic divergence in transplantation : with special reference to MHC genes and HLA typing

    Detta är en avhandling från Stockholm : Karolinska Institutet, Biosciences and Nutrition

    Författare :Henrik Zetterquist; Karolinska Institutet.; Karolinska Institutet.; [1998]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Clinical transplantation; HLA; histocompatibility testing; PCR; allele-specific amplification; sequence-specific primers; bone marrow transplantation; mixed chimerism; minimal residual disease; acute lymphoblastic leukemia;

    Sammanfattning : Transplantation immunology basically deals with the immune mechanisms that lead to rejection of transplanted tissues. Lymphocytes are the keymediators of graft rejection. At the time for this observation, the genetic loci responsible for histocompatibility between individuals of the same species were already identified in the mouse. LÄS MER