Sökning: "sequence specific primers"
Visar resultat 1 - 5 av 32 avhandlingar innehållade orden sequence specific primers.
1. Phylogenetic Inference and Allopolyploid Speciation - A Study of Silene section Physolychnis
Sammanfattning : The major theme of this thesis is allopolyploidization, with focus on Silene section Physolychnis. The evolutionary history of an allopolyploid species can be established by inferring the sister relationships of the homoeologous sequences from low copy nuclear markers. LÄS MER
2. Selection of antigens for antibody-based proteomics
Sammanfattning : The human genome is predicted to contain ~20,500 protein-coding genes. The encoded proteins are the key players in the body, but the functions and localizations of most proteins are still unknown. LÄS MER
3. Arrayed identification of DNA signatures
Sammanfattning : In this thesis techniques are presented that aim to determine individual DNA signatures by controlled synthesis of nucleic acid multimers. Allele-specific extension reactions with an improved specificity were applied for several genomic purposes. Since DNA polymerases extend some mismatched 3’-end primers, an improved specificity is a concern. LÄS MER
4. Fatty Acyl Reductases and Fatty Acyl Desaturases. Deciphering the biosynthesis of species-specific moth female sex pheromones from common fatty acids
Sammanfattning : Moths (Lepidoptera) are dependent on female produced pheromones, chemical signals, for attracting a mate. Pheromones are most commonly made up by saturated and/or unsaturated fatty acid derived alcohols, acetates, and/or aldehydes that are produced by a set of enzymes in the female pheromone gland. LÄS MER
5. The use of molecular techniques for identification of genetic divergence in transplantation : with special reference to MHC genes and HLA typing
Sammanfattning : Transplantation immunology basically deals with the immune mechanisms that lead to rejection of transplanted tissues. Lymphocytes are the key mediators of graft rejection. At the time for this observation, the genetic loci responsible for histocompatibility between individuals of the same species were already identified in the mouse. LÄS MER