Sökning: "sensitive detection of pathology"

Visar resultat 1 - 5 av 15 avhandlingar innehållade orden sensitive detection of pathology.

1. 1. Genotyping and Mutation Detection In Situ : Development and application of single-molecule techniques

   Författare :Ida Grundberg; Mats Nilsson; Ulf Landegren; Olli-Pekka Kallioniemi; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; padlock probes; in situ; rolling circle amplification; mRNA; genotyping; mutation detection; cancer; tissue sections; diagnostics; single-molecule; single-cell; microscopy; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Molekylär medicin; Molecular Medicine;

   Sammanfattning : The human body is composed of trillions of cells closely working together to maintain a functional organism. Every cell is unique in molecular composition and can acquire genetic variations that might cause it to turn pathological. LÄS MER

3. 2. Detection of immunoglobulin heavy chain gene rearrangement with PCR for MRD analysis in lymphoproliferative disorders

   Författare :Ulf Thunberg; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Minimal residual disease; Immunoglobulin gene rearrangement; PCR; Leukemia; Lymphoma; Myeloma; Genetik; Clinical genetics; Klinisk genetik; Pathology; patologi;

   Sammanfattning : Immunoglobulin heavy chain (IGH) gene rearrangement occurs during early B-lymphocyte differentiation, assembling the different IGH gene segments to a functional gene, which can serve as a marker for study of lineage association and detection of Minimal Residual Disease (MRD) in clonal diseases deriving from B-lymphocytes or their early differentiation stages. Use of a molecular marker for the leukemic cells could help improve treatment by monitoring therapeutic efficacy, predicting relapse, and identifying very small amounts of tumour cells contaminating autografts after purging or enrichment of stem cells. LÄS MER

4. 3. Ex‘PLA’ining the progression of pathological proteins in Alzheimer’s and Parkinson’s diseases : see(d)ing is believing

   Författare :Anish Behere; Joakim Bergström; Sara Ekmark-Lewén; Martin Ingelsson; Stina Syvänen; Hilal Lashuel; Uppsala universitet; []
   Nyckelord :Alpha synuclein; Tau; Proteoforms; Phosphorylation; Oligomers; Proximity Ligation Assay; sensitive detection of pathology; Alzheimer s disease; Parkinson s disease; pS129; pS202 pT205; pT231; PFFs;

   Sammanfattning : Alzheimer’s disease (AD) and Parkinson’s disease (PD) are the two most common forms of neurodegenerative disorders affecting approximately 50 million people worldwide. The underlying neuropathological processes leading to AD and PD share many similarities, i.e. aberrant protein aggregation of tau and alpha-synuclein (αSyn) in the brain. LÄS MER

5. 4. Minimal Residual Disease Assessment in Childhood Acute Lymphoblastic Leukemia

   Författare :Ingrid Thörn; Christer Sundström; Richard Rosenquist; Gisela Barbany; Johan Botling; Kjeld Schmiegelow; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Childhood acute lymphoblastic leukemia; minimal residual disease; IG TCR gene rearrangements; BCR-ABL1 fusion gene transcripts; real-time quantitative PCR; multicolor flow cytometry; Pathology; Patologi; Pathology; patologi;

   Sammanfattning : Traditionally, response to treatment in hematological malignancies is evaluated by light microscopy of bone marrow (BM) smears, but due to more effective therapies more sensitive methods are needed. Today, detection of minimal residual disease (MRD) using immunological and molecular techniques can be 100 times more sensitive than morphology. LÄS MER

7. 5. Proximity Ligation as a Universal Protein Detection Tool

   Författare :Mats Gullberg; Charles Cantor; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; Proximity ligation; proteomics; aptamer; antibody; molecular beacon; rolling circle amplification; Molekylärmedicin; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Molekylär medicin; Molecular Medicine;

   Sammanfattning : Among the great challenges in biology are the precise quantification of specific sets of proteins and analyses of their patterns of interaction on a much larger scale than is possible today.This thesis presents a novel protein detection technique - proximity ligation - and reports the development and application of a nucleic acid amplification technique, RCA. LÄS MER