Sökning: "sandwich immunoassay"

Visar resultat 1 - 5 av 13 avhandlingar innehållade orden sandwich immunoassay.

  1. 1. Biomarker detection via lab-on-a-chip integrated immunoaffinity approach for fluorescence and mass spectrometry readout

    Författare :Asilah Ahmad Tajudin; Avdelningen för Biomedicinsk teknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; immunoaffinity; affinity prefractionation; immunoMALDI; solid-phase extraction; protein digestion; ISET; antibody microarray; signal amplification; fluorescence; microfluidics; lab on a chip; separation; acoustophoresis; ultrasound; acoustic radiation force; plasma; proteomics; MALDI mass spectrometry; immunoassay; porous silicon.;

    Sammanfattning : The blood/plasma protein biomarker profile represents the pathological and physiological changes relating to human diseases. However, the plasma proteome reflects a high degree of complexity that results in a challenge for most analytical methods in clinical diagnostics. LÄS MER

  3. 2. Detection of Bio-analytes with Streaming Current : From Fundamental Principles to Novel Applications

    Författare :Siddharth S. Sahu; Apurba Dev; Shi-Li Zhang; Michel Calame; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Streaming current; Electrokinetic biosensor; Size and Charge effects; membrane protein profiling of extracellular vesicles; sandwich immunoassay; charge-labelled antibodies; multiplexed detection; exosome heterogeneity;

    Sammanfattning : A biosensor based on streaming current is a new and relatively unexplored subject with significant potential. This thesis attempts to gain a deeper understanding of the governing principles, and then exploit them to further improve its performance as well as develop novel applications. LÄS MER

  4. 3. Proteomic strategies for blood biomarker development in rare dystrophinopathies

    Författare :Camilla Johansson; Cristina Al-Khalili Szigyarto; Ola Söderberg; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Duchenne muscular dystrophy; Becker muscular dystrophy; proteomics; affinity-proteomics; biomarkers; DIA-MS; SRM-MS; immunoassay; suspension bead arrays; Duchenne muskeldystrofi; Becker muskeldystrofi; proteomik; affinitetsproteomik; biomarkörer; DIA-MS; SRM-MS; immunoassay; suspension bead array; Biotechnology; Bioteknologi;

    Sammanfattning : Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD) are two rare genetic disorders of the family dystrophinopathy. They are both caused by the lack of, or reduced production of, the protein dystrophin. LÄS MER

  5. 4. Design of a rapid point-of-care immunoassay : Based on a single-step non-competitive immunoassay with magnetic permeability detection

    Författare :Filiz Ibraimi; Tillämpad biokemi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Superparamagnetic nanoparticles; magnetic permeability; two-site immunoassay; point-of-care; diagnostic test; biomarkers;

    Sammanfattning : This thesis describes the development of a one-step, specific, cost-effective and rapid in vitro point-of-care immunoassay test based on a unique magnetic immunoassay principle. The principle in question is based on a non-competitive heterogeneous immunoassay in a one-step analysis, relying on magnetic permeability detection. LÄS MER

  7. 5. New Bioanalytical Approaches, Magneto Immunoassays and SIRE Technology Based Biosensors

    Författare :Kirstin Kriz; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemical technology; Biokemisk teknik; antigens; superparamagnetic nanoparticles; magnetic inductance transducer; competitive magneto immunoassay; enzymes; metabolites; differential measurements; SIRE; biosensor;

    Sammanfattning : This thesis presents two new technologies, SIRE technology based biosensors and magneto immunoassays, which can be used for bioanalytical applications. One of the main problems associated with biosensor technology is the instability of the recognition element since it is of biological origin and thus, has a limited lifetime. LÄS MER